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#9904 Rapamycin

  • Rapamycin Data Sheet PDF
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希望納入価格 (円)
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2019年12月16日15時25分 現在
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#9904S10 nmol13,000
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概要
FRAP/mTOR タンパク質の活性を抑制します
分子量 
914.2
分子の化学式
C51H79NO13
貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with serum in the presence of the indicated concentrations of Rapamycin, using Phospho-p70 S6 Kinase (Thr389) Antibody #9205 (upper), total p70 S6 kinase antibody #9202 (middle) or Phospho-p44/42 MAPK (Thr202/Tyr204) Antibody #9101 (lower).

Structure

Structure
バックグラウンド

Rapamycin is a bacterial macrolide with antifungal and immunosuppressant activities (1). Rapamycin forms a complex with the immunophilin FKBP12 which then inhibits the activity of FRAP/ mTOR (TOR in yeast) (2,3). Rapamycin treatment of cells leads to the dephosphorylation and inactivation of p70 S6 kinase. Rapamycin also leads to the dephosphorylation of 4E-BP1/PHAS1, thereby promoting its binding to and inactivation of eIF4E (4,5). This activity has been shown to be the basis for Rapamycin's ability to block protein synthesis and to arrest cell cycle progression in the G1-phase (6,7). However, it has been suggested that Rapamycin's inhibition of the G1/S transition may be the consequence of its effect on cyclin D1 mRNA and protein stability (8).

使用文献
 
関連製品
12611   DMSO (Dimethyl Sulfoxide), Sterile
2217   S6 Ribosomal Protein (5G10) Rabbit mAb
2317   S6 Ribosomal Protein (54D2) Mouse mAb
4858   Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb
9206   Phospho-p70 S6 Kinase (Thr389) (1A5) Mouse mAb
9234   Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb
9451   Phospho-4E-BP1 (Ser65) Antibody
9455   Phospho-4E-BP1 (Thr70) Antibody
9459   Phospho-4E-BP1 (Thr37/46) Antibody
9918   Translational Control Antibody Sampler Kit

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Rapamycin

Immune Cell Signaling Pathways

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