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#97971 HLA-DRA (E9R2Q) XP® Rabbit mAb

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希望納入価格 (円)
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2019年11月13日11時50分 現在
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#97971S100 μL62,000
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#97971T20 μL39,000
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HLA-DRA 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性30-40Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト -
97971 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫組織染色 (パラフィン) (1:125-1:500)Immunohistochemistry (Leica® Bond™) (1:50-1:200)

下記ステップについては、データシートの右側もあわせてご参照ください。

IHC-P: 抗体希釈液 / 抗原賦活化

CST推奨プロトコールに欠かせない関連製品

特異性・感度
HLA-DRA (E9R2Q) XP® Rabbit mAb recognizes endogenous levels of total HLA-DRA protein.
使用抗原
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val153 of human HLA-DRA protein.

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社内データ

※下記の社内データは、すべて97971 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected (+) with constructs expressing full-length human Myc/DDK-tagged HLA-DRA (hHLA-DRA-Myc/DDK), HLA-DPA1 (hHLA-DPA1-Myc/DDK), HLA-DOA (hHLA-DOA-Myc/DDK), HLA-DQA1 (hHLA-DQA1-MycDDK), HLA-DQA2 (hHLA-DQA2-Myc/DKK), or HLA-DMA (hHLA-DMA-Myc/DKK), using HLA-DRA (E9R2Q) XP® Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HLA-DRA (E9R2Q) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

IHC-Leica® Bond™

IHC-Leica® Bond™

Immunohistochemical analysis of paraffin-embedded human bladder adenocarcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb performed on the Leica® BOND™ Rx.


IHC-Leica® Bond™

IHC-Leica® Bond™

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

IHC-Leica® Bond™

IHC-Leica® Bond™

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Raji cell pellet (left, positive) or Jurkat cell pellet (right, negative) using HLA-DRA (E9R2Q) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human mucoepidermoid carcinoma of the larynx using HLA-DRA (E9R2Q) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human T-cell lymphoma using HLA-DRA (E9R2Q) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded ovarian carcinoma (left) or colon carcinoma (right) using HLA-DRA (E9R2Q) XP® Rabbit mAb (top) or HLA-DRA Rabbit mAb (bottom). These two antibodies detect independent, unique epitopes on human HLA-DRA. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using HLA-DRA (E9R2Q) XP® Rabbit mAb.


バックグラウンド

Major histocompatibility complex class II (MHC class II) molecules are heterodimeric, transmembrane glycoproteins expressed on the surface of antigen-presenting cells such as macrophages, dendritic cells, and B cells. Expression can also be induced on other cell types through interferon-γ signaling (1). Prior to being displayed on the cell membrane, MHC class II molecules are loaded with exogenous peptide antigens approximately 15-24 amino acids in length that were derived from endocytosed extracellular proteins digested in the lysosome (2). Antigen-presentation through MHC class II is required for T cell activation during the immune response to extracellular pathogens (2). In humans, the MHC class II protein complex is encoded by the human leukocyte antigen gene complex (HLA). HLAs corresponding to MHC class II are HLA-DP, HLA-DM, HLA-DOA, HLA-DOB, HLA-DQ, and HLA-DR (3).

使用例
 
関連製品
13079   Immunohistochemistry Application Solutions Kit (Rabbit)
14166   Hematoxylin
14177   SignalStain® Mounting Medium
14746   SignalStain® Citrate Unmasking Solution (10X)
15019   Animal-Free Blocking Solution (5X)
7003   20X LumiGLO® Reagent and 20X Peroxide
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8059   SignalStain® DAB Substrate Kit
8112   SignalStain® Antibody Diluent
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)

BOND is a trademark of Leica Biosystems Melbourne Pty. Ltd. No affiliation or sponsorship between CST and Leica Microsystems IR GmbH or Leica Biosystems Melbourne Pty. Ltd is implied.
Tween is a registered trademark of ICI Americas, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
LEICA is a registered trade​mark of Leica Microsystems IR GmbH.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

HLA-DRA (E9R2Q) XP® Rabbit mAb

Immune Cell Signaling Pathways

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