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#9727 Tri-Methyl-Histone H3 (Lys4) Antibody

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希望納入価格 (円)
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2020年2月28日15時15分 現在
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#9727S100 μL67,000
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#9727L300 μL156,000

HISTONE H3 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性17Rabbit-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト、マウス、ラット、サル アフリカツメガエル、ゼブラフィッシュ
9727 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫組織染色 (パラフィン) (1:50)免疫蛍光細胞染色 (IF-IC) (1:1000)クロマチン免疫沈降 (1:50)

下記ステップについては、データシートの右側もあわせてご参照ください。

IHC-P: 抗体希釈液 / 抗原賦活化

CST推奨プロトコールに欠かせない関連製品

特異性・感度
内在性レベルのLys4 がトリメチル化されたHistone H3 タンパク質を検出します。Lys4 がジメチル化されたHistone H3 タンパク質と若干交差しますが、Lys4が非メチル化およびモノメチル化されたHistone H3 タンパク質とは交差しません。また、Lys9、Lys27 およびLys36 がメチル化されたHistone H3 タンパク質およびLys20 がメチル化されたHistone H4 タンパク質とも交差しません。
使用抗原
Lys4 がトリメチル化されたHistone H3 タンパク質のN末端領域 (合成ペプチド)

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社内データ

※下記の社内データは、すべて9727 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of lysates from HeLa, NIH/3T3, C6 and COS cells, using Tri-Methyl-Histone H3 (Lys4) antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon using Tri-Methyl-Histone H3 (K4) Rabbit Antibody in the presence of non-methyl peptide (left) or K4 tri-methyl peptide (right).

IF-IC

IF-IC

Confocal immunofluorescent analysis of NIH/3T3 cells labeled with Tri-Methyl-Histone H3 (Lys4) Antibody (green, left) compared to an isotype control (right). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).


Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Tri-Methyl-Histone H3 (Lys4) Antibody or Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by Real-Time PCR, using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

ELISA

ELISA

Tri-Methyl-Histone H3 (Lys4) Antibody specificity was determined by peptide ELISA. Each graph depicts a titration of this antibody and the corresponding reactivity toward the non-methyl, mono-methyl, di-methyl and tri-methyl states of the indicated histone H3 or H4 lysine residue.

バックグラウンド

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker (9).

使用例
 
関連製品
1064   Tri-Methyl-Histone H3 (Lys4) Blocking Peptide
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
13079   Immunohistochemistry Application Solutions Kit (Rabbit)
14166   Hematoxylin
14177   SignalStain® Mounting Medium
14746   SignalStain® Citrate Unmasking Solution (10X)
15019   Animal-Free Blocking Solution (5X)
2650   Histone H3 Antibody (ChIP Formulated)
4499   Histone H3 (D1H2) XP® Rabbit mAb
4620   Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated)
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9701   Phospho-Histone H3 (Ser10) Antibody
9707   Methyl-Histone H3 (Arg2) Antibody
9715   Histone H3 Antibody
9717   Histone H3 (3H1) Rabbit mAb
9723   Mono-Methyl-Histone H3 (Lys4) Antibody
9725   Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)

Alexa Fluor is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Tri-Methyl-Histone H3 (Lys4) Antibody

Metabolic Reprogramming in Disease

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