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#9706 Phospho-Histone H3 (Ser10) (6G3) Mouse mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年12月13日15時35分 現在
ご登録代理店情報 i
#9706S100 μL66,000
#9706L300 μL152,000

HISTONE H3 XP®モノクローナル抗体 | HISTONE H3 XP®モノクローナル抗体 | HISTONE H3 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性17Mouse IgG1-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス、ラット -
9706 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫蛍光細胞染色 (IF-IC) (1:400)免疫蛍光染色 (IF-F) (1:100)フローサイトメトリー (1:25)


内在性レベルのSer10 がリン酸化されたHistone H3 タンパク質を検出します。他のリン酸化Histone タンパク質やアセチル化Histone H3 タンパク質とは交差しません。
ヒトのHistone H3 タンパク質のSer10 周辺領域 (合成リン酸化ペプチド)

ホモロジー (相同性) 検索をご希望の場合 >>>



※下記の社内データは、すべて9706 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of whole cell lysates from NIH/3T3 cells, untreated or treated with serum plus calyculin A (to induce phosphorylation of H3), using Phospho-Histone H3 (Ser10) (6G3) Mouse mAb (top), Phospho-Histone H3 (Ser10) Antibody #9701 (middle) or Histone H3 Antibody #9712 (bottom).



Confocal immunofluorescent analysis of proliferating/mitotic cells labeled with Phospho-Histone H3 (Ser10) (6G3) Mouse mAb (blue) in the subventricular zone following 4 h reperfusion after cerebral ischemia. Red = EGR1 antibody #4152. Green = Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854.



Confocal immunofluorescent analysis of NIH/3T3 cells labeled with Phospho-Histone H3 (Ser10) (6G3) Mouse mAb (red) and alpha/beta-Tubulin Antibody #2148 (green) showing different stages of the cell cycle. Nonmitotic (A), prophase (B), metaphase (C) and anaphase (D).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of pacilitaxel-treated THP1 cells, using Phospho-Histone H3 (Ser10) (6G3) Mouse mAb versus propidium iodide (DNA content). The red population indicates positive Phospho-Histone H3 cells.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Phospho-Histone H3 (Ser10) (6G3) Mouse mAb staining of untreated (blue) or serum/calyculin-treated (green) Ramos cells compared to a nonspecific negative control antibody (red).


Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
14269   Histone H3 (1B1B2) Mouse mAb
3638   Histone H3 (96C10) Mouse mAb
3680   Histone H3 (96C10) Mouse mAb (IHC Formulated)
6883   SignalFire™ ECL Reagent
7076   Anti-mouse IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9701   Phospho-Histone H3 (Ser10) Antibody
9711   Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody
9715   Histone H3 Antibody

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


Phospho-Histone H3 (Ser10) (6G3) Mouse mAb

Metabolic Reprogramming in Disease