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#9701 Phospho-Histone H3 (Ser10) Antibody

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年12月13日15時35分 現在
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#9701S100 μL66,000
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#9701L300 μL152,000

HISTONE H3 XP®モノクローナル抗体 | HISTONE H3 XP®モノクローナル抗体 | HISTONE H3 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性17Rabbit-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト、マウス、ラット、サル、キイロショウジョウバエ、出芽酵母 アフリカツメガエル
9701 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫組織染色 (パラフィン) (1:200)免疫組織染色 (凍結) (1:400)免疫蛍光細胞染色 (IF-IC) (1:800)フローサイトメトリー (1:50)

下記ステップについては、データシートの右側もあわせてご参照ください。

IHC-F: 固定

IHC-P: 抗体希釈液 / 抗原賦活化

CST推奨プロトコールに欠かせない関連製品

特異性・感度
内在性レベルのSer10 がリン酸化されたHistone H3 タンパク質を検出します。他のリン酸化あるいはアセチル化Histone タンパク質とは交差しません。
使用抗原
ヒトのHistone H3 タンパク質のSer10 周辺領域 (合成リン酸化ペプチド)

ホモロジー (相同性) 検索をご希望の場合 >>>

ホモロジー検索をご要望の際は、ご希望のサンプル種のアミノ酸配列とともにお問合せください。

社内データ

※下記の社内データは、すべて9701 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of whole cell lysates of NIH/3T3 cells, untreated, treated with TSA (to induce histone acetylation), serum plus calyculin A (to induce phosphorylation of H3) or both, using Phospho-Histone H3 (Ser10) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical staining of phosphorylated histone H3 in paraffin-embedded human breast carcinoma showing nuclear localization using Phospho-Histone H3 (Ser10) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of HT29 cells using Phospho-Histone H3 (Ser10) Antibody. Note the specific staining of mitotic cells.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-Histone H3 (Ser10) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using Phospho-Histone H3 (Ser10) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor using Phospho-Histone H3 (Ser10) Antibody #9701.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-Histone H3 (Ser10) Antibody in the presence of control peptide (left) or Phospho-Histone H3 (Ser10) Blocking Peptide #1000 (right).

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen H1650 xenograft, showing staining of mitotic cells using Phospho-Histone H3 (Ser10) Antibody.

IF-IC

IF-IC

Confocal microscopic image of a mitotic HeLa cell labeled with Phospho-Histone H3 (Ser10) Antibody (red) and Survivin (6E4) Mouse mAb #2802 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells, using Phospho-Histone H3 (Ser10) Antibody versus propidium iodide (DNA content). The box indicates phospho-histone H3 positive cells.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Phospho-Histone H3 (Ser10) Antibody staining of untreated (blue) or serum/calyculin treated (green) Ramos cells compared to a nonspecific negative control antibody (red).

Dot Blot-Peptide

Dot Blot-Peptide

Peptide dot blot analysis demonstrating Phospho-Histone H3 (Ser10) Antibody #9701 antibody specificity. Antibody binding to pre-coated histone H3 peptides is shown using Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb #53348,

Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb #3377, and Phospho-Histone H3 (Ser10) Antibody #9701. As shown, Phospho-Histone H3 (Ser10) Antibody #9701 detects histone H3 phosphorylated on Ser10; however, this antibody does not detect histone H3 phosphorylated on Ser10 when Lys9 is acetylated or methylated. In addition, this antibody does not cross-react with histone H3 phosphorylated on Ser28.


バックグラウンド

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

使用例
 
関連製品
1000   Phospho-Histone H3 (Ser10) Blocking Peptide
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2650   Histone H3 Antibody (ChIP Formulated)
3377   Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb
4499   Histone H3 (D1H2) XP® Rabbit mAb
4620   Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated)
53348   Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8112   SignalStain® Antibody Diluent
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
9441   Acetylated-Lysine Antibody
9681   Acetylated-Lysine Mouse mAb (Ac-K-103)
9711   Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody
9715   Histone H3 Antibody
9717   Histone H3 (3H1) Rabbit mAb

DRAQ5 is a registered trademark of Biostatus Limited.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Phospho-Histone H3 (Ser10) Antibody

Immune Cell Signaling Pathways

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