#9614 Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb |
CSTコード | 包装 | 希望納入価格 (円) |
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#9614S | 100 μL | 66,000 | ログインすると国内在庫状況がご確認いただけます。
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#9614L | 300 μL | 152,000 |
AKT 製品一覧 | Motif 抗体比較 | #9614 が入っているAntibody Sampler キット一覧
感度 | 抗体の由来 | 貯法 | |
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内在性 | Rabbit IgG | -20℃ |
種交差性 (社内試験済) | |
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ヒト、マウス、キイロショウジョウバエ、すべての種 | - |
ウェスタンブロッティング (1:1000)、免疫沈降 (1:50)、免疫組織染色 (パラフィン) (1:1000)、ELISA-P (1:1000)
下記ステップについては、データシートの右側もあわせてご参照ください。

IHC-P: 抗体希釈液 / 抗原賦活化
特異性・感度 | |
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-3 の位置にアルギニンが存在するリン酸化セリン/スレオニンを含むペプチドとタンパク質を検出します。-5 と-3 の位置にアルギニンが存在するリン酸化セリン/スレオニンを持つペプチドを優先的に認識する場合があります。 |
使用抗原 | |
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リン酸化Akt の基質ペプチド |
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モチーフ | |
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RXXS*/T* |
社内データ |
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※下記の社内データは、すべて9614 の推奨プロトコールで実験した結果です。
ELISA-Peptide

Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* and S* denote phosphorylated threonine and serine.)
Western Blotting

Western blot analysis of extracts from serum-starved NIH/3T3 cells, phosphorylated in vitro with Akt kinase, or treated in culture with PDGF or FBS/Calyculin A, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb.
IP

Immunoprecipitation of extracts from serum-starved NIH/3T3 cells, untreated or treated with FBS/Calyculin A, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb, followed by Western blot analysis using the same antibody.
IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or lambda phosphatase-treated (right), using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb.
IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded lung carcinoma, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb.
IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded renal cell carcinoma, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb.
バックグラウンド |
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An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery.
- Montminy, M. (1997) Annu Rev Biochem 66, 807-22.
- Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
- Marte, B.M. and Downward, J. (1997) Trends Biochem Sci 22, 355-8.
- Jiang, B.H. et al. (2000) Proc Natl Acad Sci USA 97, 1749-53.
- Scheid, M.P. and Woodgett, J.R. (2000) Curr Biol 10, R191-4.
- Alessi, D.R. et al. (1996) FEBS Lett 399, 333-8.
使用例 |
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Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
本製品は試験研究用です。