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#9371 Phospho-PKC (pan) (βII Ser660) Antibody

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2020年2月26日16時0分 現在
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#9371S100 μL67,000
#9371T20 μL39,000
Custom Antibody Sampler Kitの構成品を選択できます。

PKCPAN 製品一覧 | #9371 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
内在性78, 80, 82, 85Rabbit-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス、ラット、サル -
9371 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)


内在性レベルの、PKC βⅡ タンパク質のC末端側のSer660 と一致する部位がリン酸化されたPKC タンパク質の α、βI, βⅡ, δ, ε, ηおよびθ アイソフォームを検出します。他の部位がリン酸化されたPKC タンパク質とは交差しません。
ヒトのPKC βⅡ タンパク質のSer660 周辺領域 (合成ペプチド)

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※下記の社内データは、すべて9371 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of Baculovirus expressed PKCβ and PKCβ Ser660/Ala mutant, using Phospho-PKC (pan) (βII Ser660) Antibody (upper) or control PKCβ antibody (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from TPA, Go6983 and/or Bisindolylmaleimide treated 293 cells, using Phospho-PKC (pan) (βII Ser660) Antibody.

Western Blotting

Western Blotting

Western blot analysis of Baculovirus expressed PKC isoforms, using Phospho-PKC (pan) (βII Ser660) Antibody.


Activation of protein kinase C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction (1,2). PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG, and phorbol esters (3-5). Members of these three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to substrate-binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation occurs in vivo at Thr500 in the activation loop, at Thr641 through autophosphorylation, and at the carboxy-terminal hydrophobic site Ser660 (2). Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. The enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKCμ (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization (6). PKC-related kinases (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs, and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kinase activity (7).

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


Phospho-PKC (pan) (βII Ser660) Antibody

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