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#9309 Rb (4H1) Mouse mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2020年2月26日16時0分 現在
ご登録代理店情報 i
#9309S100 μL58,000
#9309L300 μL135,000
#9309T20 μL39,000
Custom Antibody Sampler Kitの構成品を選択できます。

RB 製品一覧 | #9309 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
内在性110Mouse IgG2a-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、サル、ウシ、ブタ -
9309 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:2000)免疫沈降 (1:100)免疫組織染色 (パラフィン) (1:400-1:1600)免疫蛍光細胞染色 (IF-IC) (1:800-1:3200)フローサイトメトリー (1:200-1:800)クロマチン免疫沈降 (1:200)


IHC-P: 抗体希釈液 / 抗原賦活化


内在性レベルのRb タンパク質を検出します。Rb ホモログ (p107、p130)、及び他のタンパク質とは交差しません。
ヒトのRb タンパク質の701-928番目の残基を含むRb-C 融合タンパク質。エピトープは、ヒトのRb タンパク質のHis890 周辺領域にあります。

ホモロジー (相同性) 検索をご希望の場合 >>>



※下記の社内データは、すべて9309 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence® Rb siRNA I #6451 (+) or SignalSilence® Rb siRNA II (+), using Rb (4H1) Mouse mAb #9309 and α-Tubulin (11H10) Rabbit mAb #2125. The Rb (4H1) Mouse mAb confirms silencing of Rb expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Rb siRNA.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, untreated or hydroxyurea-treated (G1/S), using Rb (4H1) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using Rb (4H1) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Rb (4H1) Mouse mAb.



Confocal immunofluorescent image of SH-SY5Y cells, using RB (4H1) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, using Rb (4H1) Mouse mAb versus propidium iodide (DNA content). The box indicates Rb positive cells.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from Raji cells and either Rb (4H1) Mouse mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using using SimpleChIP® Human Timeless Intron 1 Primers #7001, human DHFR promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


The retinoblastoma tumor suppressor protein Rb regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
14166   Hematoxylin
14177   SignalStain® Mounting Medium
14746   SignalStain® Citrate Unmasking Solution (10X)
15019   Animal-Free Blocking Solution (5X)
2181   Phospho-Rb (Ser608) Antibody
6883   SignalFire™ ECL Reagent
7076   Anti-mouse IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8059   SignalStain® DAB Substrate Kit
8112   SignalStain® Antibody Diluent
8125   SignalStain® Boost IHC Detection Reagent (HRP, Mouse)
9301   Phospho-Rb (Ser795) Antibody
9303   Rb Control Proteins
9307   Phospho-Rb (Ser780) Antibody
9308   Phospho-Rb (Ser807/811) Antibody
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


Rb (4H1) Mouse mAb

Immune Cell Signaling Pathways