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#9286 Phospho-p53 (Ser15) (16G8) Mouse mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2020年2月28日15時15分 現在
ご登録代理店情報 i
#9286S100 μL67,000
#9286L300 μL156,000
#9286T20 μL39,000
Custom Antibody Sampler Kitの構成品を選択できます。

P53 製品一覧 | #9286 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
内在性53Mouse IgG1-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト -
9286 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫蛍光細胞染色 (IF-IC) (1:400)フローサイトメトリー (1:1600)


内在性レベルのSer15 がリン酸化されたp53 タンパク質を検出します。他の部位がリン酸化されたp53 タンパク質とは交差しません。
ヒトのp53 タンパク質のSer15 周辺領域 (合成リン酸化ペプチド)

ホモロジー (相同性) 検索をご希望の場合 >>>



※下記の社内データは、すべて9286 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from HT29 cells, untreated or UV-treated (100 mJ/cm2, 1 hr), using Phospho-p53 (Ser15) (16G8) Mouse mAb (upper) or p53 (DO-7) Mouse mAb #48818 (lower).



Confocal immunofluorescent analysis of HT-29 cells, untreated (left) or UV-treated (right), using Phospho-p53 (Ser15) (16G8) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HT-29 cells, untreated (blue) or UV-treated (green), using Phospho-p53 (Ser15) (16G8) Mouse mAb compared to a nonspecific negative control antibody (red).


The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3). DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2 (4). MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation (5,6). p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage (4,7). Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability, and activity (8,9). p53 is phosphorylated at Ser392 in vivo (10,11) and by CAK in vitro (11). Phosphorylation of p53 at Ser392 is increased in human tumors (12) and has been reported to influence the growth suppressor function, DNA binding, and transcriptional activation of p53 (10,13,14). p53 is phosphorylated at Ser6 and Ser9 by CK1δ and CK1ε both in vitro and in vivo (13,15). Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis (16). Acetylation of p53 is mediated by p300 and CBP acetyltransferases. Inhibition of deacetylation suppressing MDM2 from recruiting HDAC1 complex by p19 (ARF) stabilizes p53. Acetylation appears to play a positive role in the accumulation of p53 protein in stress response (17). Following DNA damage, human p53 becomes acetylated at Lys382 (Lys379 in mouse) in vivo to enhance p53-DNA binding (18). Deacetylation of p53 occurs through interaction with the SIRT1 protein, a deacetylase that may be involved in cellular aging and the DNA damage response (19).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
18032   p53 (DO-1) Mouse mAb
2524   p53 (1C12) Mouse mAb
48818   p53 (DO-7) Mouse mAb
6883   SignalFire™ ECL Reagent
7076   Anti-mouse IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8514   Phospho-p53 (Ser15) (16G8) Mouse mAb (PE Conjugate)
9281   Phospho-p53 (Ser392) Antibody
9282   p53 Antibody
9284   Phospho-p53 (Ser15) Antibody
9285   Phospho-p53 (Ser6) Antibody
9287   Phospho-p53 (Ser20) Antibody
9288   Phospho-p53 (Ser9) Antibody
9289   Phospho-p53 (Ser37) Antibody

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


Phospho-p53 (Ser15) (16G8) Mouse mAb

Immune Cell Signaling Pathways