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#9282 p53 Antibody

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年12月16日15時25分 現在
ご登録代理店情報 i
#9282S100 μL56,000
#9282T20 μL39,000
Custom Antibody Sampler Kitの構成品を選択できます。

P53 製品一覧

感度分子量 (kDa)抗体の由来貯法
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、サル -
9282 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:100)クロマチン免疫沈降 (1:100)


内在性レベルのp53 タンパク質を検出します。p53 のN末端とDNA 結合領域の複数のエピトープに結合します。他のp53 関連タンパク質とは交差しません。
ヒトの全長p53 融合タンパク質

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※下記の社内データは、すべて9282 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of cell extracts using p53 Antibody.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from HCT116 cells treated with UV (100 J/m2 followed by a 3 hour recovery) and either p53 Antibody or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human CDKN1A Promoter Primers #6449, human MDM2 intron 2 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3). DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2 (4). MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation (5,6). p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage (4,7). Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability, and activity (8,9). p53 is phosphorylated at Ser392 in vivo (10,11) and by CAK in vitro (11). Phosphorylation of p53 at Ser392 is increased in human tumors (12) and has been reported to influence the growth suppressor function, DNA binding, and transcriptional activation of p53 (10,13,14). p53 is phosphorylated at Ser6 and Ser9 by CK1δ and CK1ε both in vitro and in vivo (13,15). Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis (16). Acetylation of p53 is mediated by p300 and CBP acetyltransferases. Inhibition of deacetylation suppressing MDM2 from recruiting HDAC1 complex by p19 (ARF) stabilizes p53. Acetylation appears to play a positive role in the accumulation of p53 protein in stress response (17). Following DNA damage, human p53 becomes acetylated at Lys382 (Lys379 in mouse) in vivo to enhance p53-DNA binding (18). Deacetylation of p53 occurs through interaction with the SIRT1 protein, a deacetylase that may be involved in cellular aging and the DNA damage response (19).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2521   Phospho-p53 (Ser46) Antibody
2524   p53 (1C12) Mouse mAb
2525   Acetyl-p53 (Lys382) Antibody
2526   Phospho-p53 (Ser33) Antibody
2528   Phospho-p53 (Ser315) Antibody
2570   Acetyl-p53 (Lys379) Antibody
2676   Phospho-p53 (Thr81) Antibody
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9281   Phospho-p53 (Ser392) Antibody
9284   Phospho-p53 (Ser15) Antibody
9285   Phospho-p53 (Ser6) Antibody
9286   Phospho-p53 (Ser15) (16G8) Mouse mAb
9287   Phospho-p53 (Ser20) Antibody
9288   Phospho-p53 (Ser9) Antibody
9289   Phospho-p53 (Ser37) Antibody

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


p53 Antibody

Metabolic Reprogramming in Disease