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#9253 SAPK/JNK Control Cell Extracts

  • SAPK/JNK Control Cell Extracts Data Sheet PDF
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2020年2月21日15時25分 現在
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CELL EXTRACTS 製品一覧

調製方法
Nonphosphorylated SAPK/JNK Control Cell Extracts:未処理の293 細胞の溶解物
Phosphorylated SAPK/JNK Control Cell Extracts:紫外線処理した293 細胞の溶解物
貯法
-20℃
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社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, treated with or without UV light, using Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb (upper) and SAPK/JNK (56G8) Rabbit mAb (lower).

バックグラウンド

The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).

使用例
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関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
4668   Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb
4671   Phospho-SAPK/JNK (Thr183/Tyr185) (98F2) Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9251   Phospho-SAPK/JNK (Thr183/Tyr185) Antibody
9252   SAPK/JNK Antibody
9255   Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb
9258   JNK2 (56G8) Rabbit mAb

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

SAPK/JNK Control Cell Extracts

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