CST logo

#9243 NF-κB Control Cell Extracts

  • NF-κB Control Cell Extracts Data Sheet PDF
CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年8月23日15時25分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#9243S200 μL25,000
ログインすると国内在庫状況がご確認いただけます。

CELL EXTRACTS 製品一覧

調製方法
Untreated NF-κB Control Cell Extracts:未処理のHeLa 細胞の溶解物
TNF-treated NF-κB Control Cell Extracts:hTNF-α で処理したHeLa 細胞の溶解物
貯法
-20℃
MSDSはこちらから
社内データ

Western Blotting

Western Blotting

Western blot analysis of NF-κB Control Cell Extracts #9243, using IKKα Antibody #2682 (upper), IKKβ (2C8) Rabbit mAb #2370 (middle) and Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb #2697 (lower).

Western Blotting

Western Blotting

Western blot analysis of NF-κB Control Cell Extracts #9243, using IκBα (44D4) Rabbit mAb #4812 (left) and Phospho-IκBα (Ser32) (14D4) Rabbit mAb #2859 (right).

Western Blotting

Western Blotting

Western blot analysis of NF-κB Control Cell Extracts #9243, using Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 (left) and NF-κB p65 (E498) Antibody #3987 (right).


バックグラウンド

Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2370   IKKβ (2C8) Rabbit mAb
2682   IKKα Antibody
2697   Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb
2859   Phospho-IκBα (Ser32) (14D4) Rabbit mAb
3033   Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
3035   NF-κB1 p105/p50 Antibody
3039   Phospho-NF-κB p65 (Ser468) Antibody
4806   Phospho-NF-κB p105 (Ser933) (18E6) Rabbit mAb
4812   IκBα (44D4) Rabbit mAb
4882   NF-κB2 p100/p52 Antibody
4954   RelB Antibody
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8902   Human Tumor Necrosis Factor-α (hTNF-α)

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

NF-κB Control Cell Extracts

Immune Cell Signaling Pathways

お薦めリンク

テクニカルサポート

製品特集

Applications