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#9213 p38 MAP Kinase Control Cell Extracts

  • p38 MAP Kinase Control Cell Extracts Data Sheet PDF
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2019年12月13日15時35分 現在
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CELL EXTRACTS 製品一覧

調製方法
Nonphosphorylated p38 MAP Kinase Control Cell Extracts:未処理のC-6 glioma 細胞の溶解物
Phosphorylated p38 MAP Kinase Control Cell Extracts:anisomycin 処理したC-6 glioma 細胞 の溶解物
貯法
-20℃
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社内データ

Western Blotting

Western Blotting

Western blot analysis of p38 MAPK Control Cell extracts using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb

#4511 (upper) and p38 MAPK Antibody #9212 (lower).

バックグラウンド

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).

使用例
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関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2222   Anisomycin
2307   p38γ MAPK Antibody
2308   p38δ MAPK (10A8) Rabbit mAb
2339   p38β MAPK (C28C2) Rabbit mAb
2371   p38α MAPK (7D6) Rabbit mAb
4511   Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb
4631   Phospho-p38 MAPK (Thr180/Tyr182) (12F8) Rabbit mAb
6883   SignalFire™ ECL Reagent
7727   Biotinylated Protein Ladder Detection Pack
9211   Phospho-p38 MAPK (Thr180/Tyr182) Antibody
9212   p38 MAPK Antibody
9215   Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb
9216   Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb
9217   p38α MAPK (5F11) Mouse mAb
9218   p38α MAPK Antibody
9219   Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb (Sepharose® Bead Conjugate)
9228   p38α MAPK (L53F8) Mouse mAb
9913   Phospho-p38 MAPK Pathway Sampler Kit

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

p38 MAP Kinase Control Cell Extracts

Metabolic Reprogramming in Disease

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