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#9194 p44/42 MAPK (Erk1/2) Control Cell Extracts

 
  • p44/42 MAPK (Erk1/2) Control Cell Extracts Data Sheet PDF
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希望納入価格 (円)
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2020年2月26日16時0分 現在
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#9194S150 μL26,000
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CELL EXTRACTS 製品一覧

調製方法
p44/42 MAPK (Erk1/2) Negative Control Cell Extracts: 10 μM のU0126 (#9903 MEK1/2 Inhibitor) で1時間処理したJurkat 細胞の溶解物
p44/42 MAPK (Erk1/2) Positive Control Cell Extracts: 200 nM の#4174 TPA で10分間処理したJurkat 細胞の溶解物
貯法
-20℃
MSDSはこちらから
社内データ

Western Blotting

Western Blotting

Western blot analysis of p44/42 MAPK (Erk1/2) Control Cell Extracts, treated with either U0126 or TPA, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101 (left) or p44/42 MAPK (Erk1/2) Antibody #9102 (right).

Western Blotting

Western Blotting

Western blot analysis of p44/42 MAPK (Erk1/2) Control Cell Extracts, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb #9106 (green) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (red).

バックグラウンド

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059.

使用例
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関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
4174   TPA (12-O-Tetradecanoylphorbol-13-Acetate)
4370   Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb
4376   Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb
4377   Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb
4695   p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb
4696   p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7076   Anti-mouse IgG, HRP-linked Antibody
7722   Blue Loading Buffer Pack
7723   Red Loading Buffer Pack
7727   Biotinylated Protein Ladder Detection Pack
9101   Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody
9102   p44/42 MAPK (Erk1/2) Antibody
9106   Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb
9107   p44/42 MAPK (Erk1/2) (3A7) Mouse mAb
9108   p42 MAP Kinase (Erk2) Antibody
9903   U0126
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

p44/42 MAPK (Erk1/2) Control Cell Extracts

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