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#9160 MEK1/2 Control Cell Extracts

  • MEK1/2 Control Cell Extracts Data Sheet PDF
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2019年8月16日15時20分 現在
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CELL EXTRACTS 製品一覧

調製方法
Nonphosphorylated MEK1/2 Control Cell Extracts:未処理のHeLa細胞の溶解物
Phosphorylated MEK1/2 Control Cell Extracts:TPAで処理 (200nM、15分) したHeLa細胞の溶解物
貯法
-20℃
MSDSはこちらから
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from Hela cells, untreated or TPA-treated, using Phospho-MEK1/2 (Ser217/221) (41G9) RmAb #9154 (upper) or MEK1/2 (D1A5) Rabbit mAb #8727 (lower).

バックグラウンド

MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

使用例
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2352   MEK1 (61B12) Mouse mAb
4694   MEK1/2 (L38C12) Mouse mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9121   Phospho-MEK1/2 (Ser217/221) Antibody
9122   MEK1/2 Antibody
9124   MEK1 Antibody
9125   MEK2 Antibody
9126   MEK1/2 (47E6) Rabbit mAb
9127   Phospho-MEK1 (Thr286) Antibody
9128   Phospho-MEK1 (Ser298) Antibody
9146   MEK1 (30C8) Rabbit mAb
9147   MEK2 (13E3) Rabbit mAb
9154   Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

MEK1/2 Control Cell Extracts

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