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#9158 AMPK Control Cell Extracts

  • AMPK Control Cell Extracts Data Sheet PDF
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希望納入価格 (円)
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2019年8月23日15時25分 現在
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CELL EXTRACTS 製品一覧

調製方法
Untreated AMPK Control Cell Extracts:未処理のC2C12細胞の溶解物
Treated AMPK Control Cell Extracts:血清飢餓処理したC2C12細胞の溶解物
貯法
-20℃
MSDSはこちらから
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from C2C12 cells, serum starved or serum treated for 1 hour prior to harvest, using Phospho-AMPKa (Thr172) (40H9) Rabbit mAb #2535 (upper) and AMPKa Antibody #2532 (lower).

バックグラウンド

AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits, each of which is encoded by two or three distinct genes (α1, 2; β1, 2; γ1, 2, 3) (2). The kinase is activated by an elevated AMP/ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia, and ischemia (1). The tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPKα at Thr172 in the activation loop, and this phosphorylation is required for AMPK activation (3-5). AMPKα is also phosphorylated at Thr258 and Ser485 (for α1; Ser491 for α2). The upstream kinase and the biological significance of these phosphorylation events have yet to be elucidated (6). The β1 subunit is post-translationally modified by myristoylation and multi-site phosphorylation including Ser24/25, Ser96, Ser101, Ser108, and Ser182 (6,7). Phosphorylation at Ser108 of the β1 subunit seems to be required for the activation of AMPK enzyme, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization (7). Several mutations in AMPKγ subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumulation in heart or skeletal muscle (1,2). Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS (1).

使用例
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関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2531   Phospho-AMPKα (Thr172) Antibody
2532   AMPKα Antibody
2535   Phospho-AMPKα (Thr172) (40H9) Rabbit mAb
2603   AMPKα (23A3) Rabbit mAb
3661   Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody
3662   Acetyl-CoA Carboxylase Antibody
4148   AMPKβ2 Antibody
4150   AMPKβ1/2 (57C12) Rabbit mAb
4184   Phospho-AMPKα1 (Ser485) Antibody
4185   Phospho-AMPKα1 (Ser485)/AMPKα2 (Ser491) Antibody
4186   Phospho-AMPKβ1 (Ser182) Antibody
4188   Phospho-AMPKα (Thr172) (D79.5E) Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

AMPK Control Cell Extracts

Metabolic Reprogramming in Disease

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