CST logo

#9133 Stat1/2/3/5 Control Cell Extracts

  • Stat1/2/3/5 Control Cell Extracts Data Sheet PDF
CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年8月21日15時40分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#9133S10 mini-blots25,000
ログインすると国内在庫状況がご確認いただけます。

CELL EXTRACTS 製品一覧

調製方法
Nonphosphorylated Stat3 Cell Extracts:未処理のHeLa 細胞の溶解物
Phosphorylated Stat3 Cell Extracts::血清飢餓状態でIFN-α処理したHeLa 細胞の溶解物
貯法
-20℃
MSDSはこちらから
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with IFN-alpha (100 ng/ml) for 5 min, using Stat1 (D1K9Y) Rabbit mAb #14994 (upper) and P-Stat1 (Tyr701) (58D6) Rabbit mAb #9167 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with IFN-alpha (100 ng/ml) for 5 min, using Stat3 (D3Z2G) Rabbit mAb #12640 (upper) and P-Stat3 (Tyr705) (D3A7) XP Rabbit mAb #9145 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with IFN-alpha (100 ng/ml) for 5 min, using Stat5 (D2O6Y) Rabbit mAb #94205 (upper) and P-Stat5 (Tyr694) (D47E7) Rabbit mAb #4322 (lower).


バックグラウンド

Jaks (Janus Kinases) and Stats (Signal Transducers and Activators of Transcription) are utilized by receptors for a wide variety of ligands including cytokines, hormones, growth factors and neurotransmitters. Jaks, activated via autophosphorylation following ligand-induced receptor aggregation, phosphorylate tyrosine residues on associated receptors, Stat molecules and other downstream signaling proteins (1,2). The phosphorylation of Stat proteins at conserved tyrosine residues activates SH2-mediated dimerization followed rapidly by nuclear translocation. Stat dimers bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes (1,2). The remarkable range and specificity of responses regulated by the Stats is determined in part by the tissue-specific expression of different cytokine receptors, Jaks and Stats (2,3), and by the combinatorial coupling of various Stat members to different receptors. Serine phosphorylation in the carboxy-terminal transcriptional activation domain has been shown to regulate the function of Stat1, -2, -3, -4 and -5 (1). Phosphorylation of Stat3 at Ser727 via MAPK or mTOR pathways is required for optimal transcriptional activation in response to growth factors and cytokines including IFN-gamma and CNTF (4,5). Jak/Stat pathways also play important roles in oncogenesis, tumor progression, angiogenesis, cell motility, immune responses and stem cell differentiation (6-11).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
4904   Stat3 (79D7) Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7076   Anti-mouse IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9131   Phospho-Stat3 (Tyr705) Antibody
9138   Phospho-Stat3 (Tyr705) (3E2) Mouse mAb
9139   Stat3 (124H6) Mouse mAb
9145   Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Stat1/2/3/5 Control Cell Extracts

Immune Cell Signaling Pathways

お薦めリンク

テクニカルサポート

製品特集

Applications