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#8916 Human Epidermal Growth Factor (hEGF)

  • Human Epidermal Growth Factor (hEGF) Data Sheet PDF
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希望納入価格 (円)
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2019年12月11日15時25分 現在
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#8916SC50 μg31,000
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#8916SF50 μg31,000

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EGF 製品一覧

由来
ヒトの組換えEpidermal Growth Factor (hEGF) Asn971-Arg1023 (Accession No. NM_0011963) を大腸菌発現系を用いて発現、精製したもの。
生物活性
The bioactivity of recombinant hEGF was determined in a MCF10A cell proliferation assay. The ED50 of each lot is between 10-600 pg/ml.
純度
98%以上
貯法
4℃
社内データ

Coomassie Gel

Coomassie Gel

The purity of recombinant hEGF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hEGF and staining overnight with Coomassie Blue.

Bioactivity

Bioactivity

The proliferation of MCF10A cells treated with increasing concentrations of hEGF was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hr. BrdU incorporation was determined by ELISA and the OD450-OD690 was determined.

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 cells, untreated or treated with hEGF for 10 minutes, using Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb #4407 (upper) and EGF Receptor Antibody #2232 (lower).


バックグラウンド

EGF is produced by epithelial cells, fibroblasts and many other cell types (1,2). Low molecular weight soluble EGF is generated through proteolysis of a larger ~130,000 molecular weight transmembrane precursor (1,2). Both soluble and membrane forms of EGF are active (2). EGF induces proliferation, differentiation, and survival of many cell types including tumor-derived cells (1,2, 3). There are multiple members of the EGF family and multiple members of the Erb/Her EGF receptor family. EGF binds to ErbB1/HER1 and induces homodimerization or induces heterodimerization with other Erb/Her members (1).  Binding of EGF signals through the MAPK, PI3K/Akt, and STAT 5 pathways (1). EGF, EGF family members, EGF receptors and their signaling pathways are involved in many cancers and are targets for therapeutic intervention (1, 2).

使用文献
 
関連製品
2220   Phospho-EGF Receptor (Tyr1086) Antibody
2231   Phospho-EGF Receptor (Tyr845) Antibody
2232   EGF Receptor Antibody
2234   Phospho-EGF Receptor (Tyr1068) Antibody
2235   Phospho-EGF Receptor (Tyr992) Antibody
2236   Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb
2237   Phospho-EGF Receptor (Tyr1045) Antibody
2238   Phospho-EGF Receptor (Ser1046/1047) Antibody
2239   EGF Receptor (1F4) Mouse mAb
2256   EGF Receptor (EGFR1) Mouse mAb (IP Specific)
2646   EGF Receptor (C74B9) Rabbit mAb
2963   EGF Receptor (T43) Antibody
3265   EGF Receptor (V279) Antibody
4405   EGF Receptor (15F8) Rabbit mAb
4407   Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb
8102   SignalSlide® Phospho-EGF Receptor IHC Controls
9872   Phosphate Buffered Saline (PBS-1X) pH7.2 (Sterile)
9922   Phospho-EGF Receptor Antibody Sampler Kit

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

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