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#8861 Phospho-MCM2 (Ser139) Antibody

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2020年1月24日15時25分 現在
ご登録代理店情報 i
#8861S100 μL67,000

MCM2 製品一覧

感度分子量 (kDa)抗体の由来貯法
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス、ラット、サル ハムスター、イヌ
8861 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)


内在性レベルのSer139 がリン酸化されたMCM2 タンパク質を検出します。
ヒトのMCM2 タンパク質のSer139 周辺領域 (合成ペプチド)

ホモロジー (相同性) 検索をご希望の場合 >>>



※下記の社内データは、すべて8861 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from WI-38 cells, synchronized by serum starvation, using Phospho-MCM2 (Ser139) Antibody (upper), MCM2 (D7G11) XP® Rabbit mAb #3619 (middle), and β-Actin (13E5) Rabbit mAb #4970 (lower). Cells were synchronized for 72 hours and then released by addition of serum and harvested at the times indicated.

Western Blotting

Western Blotting

Western blot analysis of extracts from U-2 OS cells, untreated (-) or treated with calf intestinal phosphatase (CIP) and λ phosphatase (+), using Phospho-MCM2 (Ser139) Antibody (upper) and MCM2 (D7G11) XP® Rabbit mAb #3619 (lower).


The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). CDK1 phosphorylation of MCM3 at Ser112 during late mitosis/early G1 phase has been shown to initiate complex formation and chromatin loading in vitro (8). Phosphorylation of MCM2 at serine 139 by cdc7/dbf4 coincides with the initiation of DNA replication (9). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,10).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
12958   Phospho-MCM2 (Ser139) (D1Z8X) XP® Rabbit mAb
2198   RPA70/RPA1 (4D9) Rat mAb
2208   RPA32/RPA2 (4E4) Rat mAb
2267   RPA70/RPA1 Antibody
3619   MCM2 (D7G11) XP® Rabbit mAb
4007   MCM2 Antibody
4012   MCM3 Antibody
4018   MCM7 Antibody
4731   ORC1 (7A7) Rat mAb
4736   ORC2 (3G6) Rat mAb
4737   ORC6 (3A4) Rat mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack

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Phospho-MCM2 (Ser139) Antibody

Immune Cell Signaling Pathways