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#8713 Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2020年1月24日15時25分 現在
ご登録代理店情報 i
#8713S100 μL67,000

PLCGAMMA1 製品一覧 | #8713 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
内在性150Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス、サル ラット
8713 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:50)免疫組織染色 (パラフィン) (1:100-1:400)免疫蛍光細胞染色 (IF-IC) (1:100-1:200)フローサイトメトリー (1:800)


IHC-P: 抗体希釈液 / 抗原賦活化


内在性レベルのSer1248 がリン酸化されたPLCγ1 タンパク質を検出します。
ヒトのPLCγ1 タンパク質のSer1248 周辺領域 (合成ペプチド)

ホモロジー (相同性) 検索をご希望の場合 >>>



※下記の社内データは、すべて8713 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved A-431 and A549 cells, untreated (-) or treated (+) with hEGF #8916 (100 ng/mL, 15 min) or serum-starved NIH/3T3 cells, untreated (-) or treated (+) with hPDGF-BB #8912 (50 ng/mL, 15 min), using Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb (upper) or PLCγ1 (D9H10) XP® Rabbit mAb #5690 (lower).



Immunoprecipitation (IP)/Western blot analysis of extracts from serum-starved HeLa cells, untreated (-) or treated (+) with TPA #4174 (100 nM, 15 min) prior to lysis in SDS (lanes 1 and 2) or IP lysis buffer (lane 3, TPA-treated only). IP Lysates were then subjected to immunoprecipitation with Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb (lane 4), PLCγ1 (D9H10) XP® Rabbit mAb #5690 (lane 5), or Normal Rabbit IgG #2729 (lane 6). The western blot was probed using Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb. Lane 3 represents 10% input.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon (normal adjacent to tumor) using Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of SignalSlide® Phospho-EGF Receptor IHC Controls #8102 [paraffin-embedded KYSE450 cell pellets untreated (left) or EGF-treated (right)] using Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb.



Confocal immunofluorescent analysis of A-431 cells, serum starved (left) or treated with hEGF #8916 (100 ng/mL for 15 min) using Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, treated with U0126 #9903 (blue) or TPA #4174 (green), using Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb.


Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (2). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (3). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (4). PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197, and 1217 is correlated with PLCγ2 activity (5,6).

Two mammalian PLCγ isoforms (γ1 and γ2) have been cloned and characterized (7,8). Like other PLC-family members, PLCγ1 and PLCγ2 contain calcium-binding (EF-hand, C2) and lipid-interacting (PH, EF-hand) domains necessary for their enzymatic activity and substrate recognition. Uniquely, PLCγ isoforms have additional, conserved SH2 and SH3 domains critical for their functions as signaling molecules and scaffolding proteins. Upon growth factor stimulation, PLCγ1 is recruited (via SH2 domains) to phosphotyrosine residues within the cytoplasmic tail of many RTKs where it serves as a substrate for the RTK and provides docking sites for additional proteins involved in RTK signaling (4-6,9-12). PLCγ1 and γ2 can also be activated downstream of receptors lacking intrinsic tyrosine kinase activity. This has been reported downstream of multiple G protein-coupled receptors and the T cell receptor in which tyrosine kinases of the Src, Syk, and Tec families serve to bind, phosphorylate, and activate PLCγ (reviewed in 13-15). Phosphorylation at tyrosine residues by both receptor and non-receptor tyrosine kinases results in robust activation of PLCγ1 activity, leading to generation of second messengers. In response to agonists, PLCγ1 is phosphorylated on Tyr783, Tyr711, and Tyr1253 (Tyr753, Tyr759, and Tyr1217 in PLCγ2) resulting in robust PI-4,5-P2 hydrolysis (4-6,9-12). Interestingly recent evidence suggests a role for tyrosine kinase-independent regulation of PLCγ in some systems. For example, in response to EGF, proline-rich regions of Akt interact with the SH3 domain of PLCγ1 resulting in association of the two enzymes, phosphorylation of PLCγ1 at Ser1248, and enhanced cellular motility (16). This finding demonstrates that PLCγ1 can function as a "scaffold" between RTKs and Akt, thereby establishing a mechanism by which the Akt signaling pathway cross-talks with tyrosine kinases. However, the mechanism and functional significance of phosphorylation at Ser1248 remains to be fully clarified, as it has also been shown that PKA-mediated phosphorylation at this site is inhibitory to PLCγ1 tyrosine phosphorylation and phospholipase activity in CD3-treated Jurkat cells (17), suggesting that Ser1248 may be an allosteric regulator of PLCγ1 activity.

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2481   Phospho-PLCbeta3 (Ser537) Antibody
2484   Phospho-PLCbeta3 (Ser1105) Antibody
2821   Phospho-PLCγ1 (Tyr783) Antibody
2822   PLCγ1 Antibody
3871   Phospho-PLCγ2 (Tyr1217) Antibody
3872   PLCγ2 Antibody
3874   Phospho-PLCγ2 (Tyr759) Antibody
4510   Phospho-PLCγ1 (Ser1248) Antibody
5690   PLCγ1 (D9H10) XP® Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8102   SignalSlide® Phospho-EGF Receptor IHC Controls
8112   SignalStain® Antibody Diluent
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
8912   Human Platelet-Derived Growth Factor BB (hPDGF-BB)
8916   Human Epidermal Growth Factor (hEGF)
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb

Metabolic Reprogramming in Disease