CST logo

#8654 Phospho-LATS1 (Thr1079) (D57D3) Rabbit mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2020年1月17日15時20分 現在
ご登録代理店情報 i
#8654S100 μL67,000

LATS 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性140Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス ラット、サル、ニワトリ、アフリカツメガエル、ゼブラフィッシュ、イヌ
8654 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)


内在性レベルのThr1079 がリン酸化されたLATS1 タンパク質を検出します。Thr1041 がリン酸化されたLATS2 タンパク質と交差すると予測されます。
ヒトのLATS1 タンパク質のThr1079 周辺領域 (合成リン酸化ペプチド)

ホモロジー (相同性) 検索をご希望の場合 >>>



※下記の社内データは、すべて8654 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or okadaic acid-treated (+), using Phospho-LATS1 (Thr1079) (D57D3) Rabbit mAb (upper) or LATS1 (C66B5) Rabbit mAb #3477 (lower).


The Large tumor suppressor (LATS) proteins (LATS1, LATS2) are serine/threonine kinases that belong to the NDR family (1). The Drosophila homolog (warts) was first identified as a tumor suppressor protein that plays a role in the maintenance of ploidy. Human LATS1 was shown to localize to the centrosome and the mitotic spindle and control G2/M transition by negatively regulating cdc2 kinase activity (2,3). LATS1 is also reported to play a role in the G1 tetraploidy checkpoint, via control of p53 expression (4). LATS1 affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1 (5). LATS1 also binds the phosphorylated form of zyxin, a regulator of actin filament assembly. This interaction promotes localization of zyxin to the mitotic spindle, suggesting a role for actin regulatory proteins during mitosis (6). Decreased expression of LATS1 is associated with breast tumor aggressiveness (7), and mutations perturbing LATS1 have been associated with human sarcomas and ovarian sarcomas (8,9). LATS1 knockout mice develop soft-tissue sarcomas, ovarian stromal cell tumor, and display a high sensitivity to carcinogenic treatments (10). LATS1 and LATS2 have also been identified as key members of the Hippo signaling pathway, a conserved kinase cascade that functions to regulate cell growth and apoptosis (11). Phosphorylation of LATS by Mammalian Sterile-20-like proteins (e.g., MST1) results in LATS-mediated phosphorylation of the transcriptional co-activators YAP and TAZ (12, 13). LATS-mediated phosphorylation of YAP and TAZ promotes their cytoplasmic sequestration and association with 14-3-3 proteins, and subsequent proteasomal degradation, leading to downregulation of YAP/TAZ target genes that promote cell growth (11, 14).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
3477   LATS1 (C66B5) Rabbit mAb
3507   Sav1 Antibody
3682   MST1 Antibody
3863   MOB1 Antibody
3952   MST2 Antibody
4911   Phospho-YAP (Ser127) Antibody
4912   YAP Antibody
5888   LATS2 (D83D6) Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8418   YAP/TAZ (D24E4) Rabbit mAb
9153   LATS1 Antibody

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


Phospho-LATS1 (Thr1079) (D57D3) Rabbit mAb

Immune Cell Signaling Pathways