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#8618 SignalSilence® CDK2 siRNA I

  • SignalSilence® CDK2 siRNA I Data Sheet PDF
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希望納入価格 (円)
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2020年2月26日16時0分 現在
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CDK2 製品一覧

種交差性機能すると推定される種
ヒト-
特異性
CDK2 タンパク質の発現を特異的に抑制します。
貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® CDK2 siRNA I (+) or SignalSilence® CDK2 siRNA II #7417 (+), using CDK2 (78B2) Rabbit mAb #2546 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The CDK2 (78B2) Rabbit mAb confirms silencing of CDK2 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

バックグラウンド

Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. Inhibitory phosphorylation occurs on Thr14 and Tyr15 (1). Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1 (2). Activation of CDK2 complexes requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160 (3), which is mediated by CAK, a complex of CDK7 and cyclin H (4). CDK2/cyclin E kinase activity is important for the G1 to S transition and phosphorylation of the Rb protein. During S-phase, active CDK2/cyclin A complexes predominate and phosphorylate E2F and the active CDK2 complex persists in the nucleus throughout G2 (5).

使用例
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関連製品
2325   Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb
2546   CDK2 (78B2) Rabbit mAb
6201   SignalSilence® Control siRNA (Fluorescein Conjugate)
6568   SignalSilence® Control siRNA (Unconjugated)
7417   SignalSilence® CDK2 siRNA II

SignalSilence is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

SignalSilence® CDK2 siRNA I

Metabolic Reprogramming in Disease

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