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#8480 β-Catenin (D10A8) XP® Rabbit mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年12月13日15時35分 現在
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#8480S100 μL61,000
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#8480T20 μL39,000
Custom Antibody Sampler Kitの構成品を選択できます。
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CATENIN-BETA 製品一覧 | β-Catenin 抗体比較 | #8480 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
内在性92Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト、マウス、ラット、サル ゼブラフィッシュ、ウシ、ブタ、モルモット、ウマ
8480 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫沈降 (1:50)免疫組織染色 (パラフィン) (1:100)免疫組織染色 (凍結) (1:100)免疫蛍光細胞染色 (IF-IC) (1:100)免疫蛍光染色 (IF-F) (1:100)フローサイトメトリー (1:50)クロマチン免疫沈降 (1:25)クロマチン免疫沈降シークエンス (1:25)

下記ステップについては、データシートの右側もあわせてご参照ください。

IHC-F: 固定

IHC-P: 抗体希釈液 / 抗原賦活化

CST推奨プロトコールに欠かせない関連製品

特異性・感度
内在性レベルのβ-Catenin タンパク質を検出します。
使用抗原
ヒトのβ-Catenin タンパク質のPro714 周辺領域 (合成ペプチド)

ホモロジー (相同性) 検索をご希望の場合 >>>

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社内データ

※下記の社内データは、すべて8480 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using β-Catenin (D10A8) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Catenin (D10A8) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using β-Catenin (D10A8) XP® Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded cell pellets, HeLa (left) or NCI-H28 (right), using β-Catenin (D10A8) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse colon using β-Catenin (D10A8) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using β-Catenin (D10A8) XP® Rabbit mAb.


IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen mouse colon using β-Catenin (D10A8) XP® Rabbit mAb.

IF-F

IF-F

Confocal immunofluorescent analysis of mouse colon using β-Catenin (D10A8) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa (left) and NCI-H28 (right) cells using β-Catenin (D10A8) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NCI-H28 cells (blue) and HeLa cells (green) using β-Catenin (D10A8) XP® Rabbit mAb (solid lines) or concentration-matched Rabbit Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as secondary antibody.

Chromatin IP-seq

Chromatin IP-seq

Chromatin immunoprecipitations were performed with cross-linked chromatin from HCT116 cells and either β-Catenin (D10A8) XP® Rabbit mAb or Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb #8814, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795. The figure shows binding across AXIN2, a known target gene of β-Catenin (see additional figure containing ChIP-qPCR data). For additional ChIP-seq tracks, please download the product data sheet.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from HCT 116 cells and either β-Catenin (D10A8) XP® Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Axin2 Intron 1 Primers #8973, SimpleChIP® Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


バックグラウンド

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

使用例
 
関連製品
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12757   SignalFire™ Elite ECL Reagent
19807   Non-phospho (Active) β-Catenin (Ser45) (D2U8Y) XP® Rabbit mAb
2009   Phospho-β-Catenin (Ser33/37) Antibody
2087   Axin1 (C76H11) Rabbit mAb
2151   Axin2 (76G6) Rabbit mAb
2230   LEF1 (C12A5) Rabbit mAb
2262   Naked1 Antibody
2286   LEF1 (C18A7) Rabbit mAb
2391   Wnt3a Antibody
2392   Wnt5a Antibody
2530   Wnt5a/b (C27E8) Rabbit mAb
2560   LRP6 (C5C7) Rabbit mAb
2568   Phospho-LRP6 (Ser1490) Antibody
2721   Wnt3a (C64F2) Rabbit mAb
2883   TCF3/TCF7L1 (D15G11) Rabbit mAb
2951   β-Catenin Antibody Sampler Kit
3195   E-Cadherin (24E10) Rabbit mAb
3216   Dvl2 Antibody
3218   Dvl3 Antibody
3224   Dvl2 (30D2) Rabbit mAb
3795   Frizzled5 Antibody
4176   Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb
4270   Non-phospho (Active) β-Catenin (Ser33/37/Thr41) Antibody
5651   Phospho-β-Catenin (Ser552) (D8E11) Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7308   PathScan® Total β-Catenin Sandwich ELISA Kit
7727   Biotinylated Protein Ladder Detection Pack
8112   SignalStain® Antibody Diluent
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
8462   CYLD (D1A10) Rabbit mAb
8814   Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb
9030   Acetyl-β-Catenin (Lys49) (D7C2) Rabbit mAb
9383   TCF/LEF Family Antibody Sampler Kit
9534   Acetyl-β-Catenin (Lys49) Antibody
9561   Phospho-β-Catenin (Ser33/37/Thr41) Antibody
9564   Phospho-β-Catenin (Ser45) Antibody
9565   Phospho-β-Catenin (Thr41/Ser45) Antibody
9566   Phospho-β-Catenin (Ser552) Antibody
9567   Phospho-β-Catenin (Ser675) Antibody
9581   β-Catenin Antibody (Amino-terminal Antigen)
9877   DDX5 (D15E10) XP® Rabbit mAb
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)

Illumina is a registered trademark of Illumina, Inc.
DRAQ5 is a registered trademark of Biostatus Limited.
SignalStain is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

β-Catenin (D10A8) XP® Rabbit mAb

Immune Cell Signaling Pathways

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