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#84192 Phospho-FoxO1 (Ser256) (E1F7T) Rabbit mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年12月6日15時40分 現在
ご登録代理店情報 i
#84192S100 μL66,000

FOXO1 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性82Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス、ラット、サル -
84192 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:50)


内在性レベルのSer256 がリン酸化されたFoxO1 タンパク質を検出します。過剰発現したSer193 がリン酸化したFoxO4 タンパク質と交差します。過剰発現したSer253 がリン酸化されたFoxO3a タンパク質と交差する可能性があります。およそ160 kDa の由来不明なタンパク質を検出します。
ヒトのFoxO1 タンパク質のSer256 周辺領域 (合成リン酸化ペプチド)

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※下記の社内データは、すべて84192 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, untreated or phosphatase-treated, using Phospho-Fox01 (Ser256) (E1F7T) Rabbit mAb (upper), FoxO1 (C29H4) Rabbit mAb #2880 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from C2C12 cells, insulin-treated (100 nM, 15 min) or LY294002-treated #9901 (50 μM, 2 hr), using Phospho-FoxO1 (Ser256) (E1F7T) Rabbit mAb (upper) or FoxO1 (C29H4) Rabbit mAb #2880 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells expressing either non-targeting shRNA (shNT) or Fox01 shRNA (shFoxO1) using Phospho-FoxO1 (Ser256) (E1F7T) Rabbit mAb (upper) and FoxO3a (75D8) Rabbit mAb #2497 (lower). 293T shNT and shFoxO1 cells were kindly provided by Dr. Anne Brunet, Stanford University, Stanford CA.


The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
12829   FoxO3a (D19A7) Rabbit mAb
13129   Phospho-FoxO3a (Ser253) (D18H8) Rabbit mAb
13953   Prestained Protein Marker, Broad Range (11-190 kDa)
14952   FoxO1 (D7C1H) Mouse mAb
2497   FoxO3a (75D8) Rabbit mAb
2599   Phospho-FoxO1 (Thr24)/FoxO3a (Thr32)/FoxO4 (Thr28) (4G6) Rabbit mAb
2880   FoxO1 (C29H4) Rabbit mAb
6883   SignalFire™ ECL Reagent
7003   20X LumiGLO® Reagent and 20X Peroxide
7074   Anti-rabbit IgG, HRP-linked Antibody
7075   Anti-biotin, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9454   FoxO1 (L27) Antibody
97635   FoxO1 (D8T1S) Mouse mAb
99199   FoxO3a (D7D3Y) Mouse mAb
9946   Forkhead Signaling Antibody Sampler Kit
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


Phospho-FoxO1 (Ser256) (E1F7T) Rabbit mAb

Immune Cell Signaling Pathways