#8418 YAP/TAZ (D24E4) Rabbit mAb
|内在性||50, 70||Rabbit IgG||-20℃|
IHC-P: 抗体希釈液 / 抗原賦活化
|内在性レベルのYAP とTAZ タンパク質を検出します。|
|ヒトのTAZ タンパク質のAsp362 周辺領域 (合成ペプチド)|
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Western blot analysis of extracts from various cell lines using YAP/TAZ (D24E4) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using YAP/TAZ (D24E4) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lymphoma using YAP/TAZ (D24E4) Rabbit mAb.
YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5). In its capacity as a transcriptional co-activator, YAP is now widely recognized as a central mediator of the Hippo Pathway, which plays a fundamental and widely conserved role in regulating tissue growth and organ size. Phosphorylation at multiple sites (e.g., Ser109, Ser127) by LATS kinases promotes YAP translocation from the nucleus to the cytoplasm, where it is sequestered through association with 14-3-3 proteins (6-8). These LATS-driven phosphorylation events serve to prime YAP for subsequent phosphorylation by CK1δ/ε in an adjacent phosphodegron, triggering proteosomal degradation of YAP (9).
YAP may also function as a transcriptional repressor (7) and is now recognized as a critical regulator of the Hippo (Mst1/Mst2) signaling pathway (8). YAP may therefore play an important role in both organ size determination and tumor suppression (8).
TAZ is a closely related transcriptional co-activator also implicated in the Hippo signaling pathway (9). Taz contains a PDZ-binding motif, which shares homology with the WW domain of YAP, and has been proposed to modulate the switch between proliferation and differentiation of mesenchymal stem cells (MSCs) via interaction with the transcription factors Runx2 and PPARγ. This process is critical for normal tissue development and tumor suppression (10). Due to its role in the determination of MSC fate, TAZ may have clinical relevance to several human diseases caused by an imbalance of MSC differentiation (11).
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