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#8418 YAP/TAZ (D24E4) Rabbit mAb

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希望納入価格 (円)
国内在庫 i
2020年2月17日11時40分 現在
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#8418S100 μL58,000
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YAP 製品一覧 | #8418 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
内在性50, 70Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト、マウス、サル -
8418 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫沈降 (1:50)免疫組織染色 (パラフィン) (1:200)

下記ステップについては、データシートの右側もあわせてご参照ください。

IHC-P: 抗体希釈液 / 抗原賦活化

CST推奨プロトコールに欠かせない関連製品

特異性・感度
内在性レベルのYAP とTAZ タンパク質を検出します。
使用抗原
ヒトのTAZ タンパク質のAsp362 周辺領域 (合成ペプチド)

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社内データ

※下記の社内データは、すべて8418 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using YAP/TAZ (D24E4) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using YAP/TAZ (D24E4) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymphoma using YAP/TAZ (D24E4) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded COS-7 cell pellets, transfected with YAP (left) or TAZ (right), using YAP/TAZ (D24E4) Rabbit mAb.

バックグラウンド

YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5). In its capacity as a transcriptional co-activator, YAP is now widely recognized as a central mediator of the Hippo Pathway, which plays a fundamental and widely conserved role in regulating tissue growth and organ size. Phosphorylation at multiple sites (e.g., Ser109, Ser127) by LATS kinases promotes YAP translocation from the nucleus to the cytoplasm, where it is sequestered through association with 14-3-3 proteins (6-8). These LATS-driven phosphorylation events serve to prime YAP for subsequent phosphorylation by CK1δ/ε in an adjacent phosphodegron, triggering proteosomal degradation of YAP (9).

YAP may also function as a transcriptional repressor (7) and is now recognized as a critical regulator of the Hippo (Mst1/Mst2) signaling pathway (8). YAP may therefore play an important role in both organ size determination and tumor suppression (8).

TAZ is a closely related transcriptional co-activator also implicated in the Hippo signaling pathway (9). Taz contains a PDZ-binding motif, which shares homology with the WW domain of YAP, and has been proposed to modulate the switch between proliferation and differentiation of mesenchymal stem cells (MSCs) via interaction with the transcription factors Runx2 and PPARγ. This process is critical for normal tissue development and tumor suppression (10). Due to its role in the determination of MSC fate, TAZ may have clinical relevance to several human diseases caused by an imbalance of MSC differentiation (11).

使用例
 
関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
13079   Immunohistochemistry Application Solutions Kit (Rabbit)
14166   Hematoxylin
14177   SignalStain® Mounting Medium
14746   SignalStain® Citrate Unmasking Solution (10X)
15019   Animal-Free Blocking Solution (5X)
3477   LATS1 (C66B5) Rabbit mAb
4883   TAZ (V386) Antibody
4911   Phospho-YAP (Ser127) Antibody
4912   YAP Antibody
5888   LATS2 (D83D6) Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8059   SignalStain® DAB Substrate Kit
8112   SignalStain® Antibody Diluent
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
9157   Phospho-LATS1 (Ser909) Antibody
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

YAP/TAZ (D24E4) Rabbit mAb

Metabolic Reprogramming in Disease

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