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#83200 AhR (D5S6H) Rabbit mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年11月12日15時40分 現在
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#83200S100 μL56,000
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AHR 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性100Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト -
83200 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫沈降 (1:100)クロマチン免疫沈降 (1:50)

CST推奨プロトコールに欠かせない関連製品

特異性・感度
内在性レベルのAhR タンパク質を検出します。
使用抗原
ヒトのAhR タンパク質のC末端近傍領域 (合成ペプチド)

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社内データ

※下記の社内データは、すべて83200 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human AhR protein (hAhR-Myc/DDK; +), using AhR (D5S6H) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using AhR (D5S6H) Rabbit mAb.

IP

IP

Immunoprecipitation of AhR from A-204 cell extracts. Lane 1 is 10% input, lane 2 is immunoprecipitation with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is immunoprecipitation with AhR (D5S6H) Rabbit mAb. Western blot analysis was performed using AhR (D5S6H) Rabbit mAb.


Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from T47D cells treated with BNF (1 μM, 45 min) and either AhR (D5S6H) XP® Rabbit mAb #83200 or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human CYP1A1 promoter primers, SimpleChIP® Human NFE2L2 Intron 1 Primers #81126, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

バックグラウンド

The aryl hydrocarbon receptor (AhR) is a ligand activated transcription factor involved in xenobiotic metabolism, cell cycle regulation, and development in response to both endogenous and environmental signals (1,2). AhR was initially identified as a receptor for dioxins, which are environmental pollutants generated by waste incineration and other industrial processes (3,4). AhR ligands include polycyclic aromatic hydrocarbons, including the carcinogen benzo(a)pyrene and other components of cigarette smoke (3,4). Naturally occurring AhR ligands include flavonoids, which are aromatic plant secondary compounds commonly found in vegetables and fruits (3). Cytoplasmic aryl hydrocarbon receptors are found in protein complexes with heat shock proteins. Upon ligand binding, AhR dissociates from heat shock proteins and translocate to the nucleus where it dimerizes with AhR nuclear translocator (ARNT, HIF-1β). The AhR/ARNT heterodimer binds to nuclear xenobiotic response elements to control the expression of genes associated with xenobiotic metabolism, including several cytochrome P450 genes (5,6). AhR is ubiquitously expressed and is thought to play a role in regulation of cell proliferation and differentiation, cytokine expression, and xenobiotic metabolism (2). Research studies link AhR activity with the control of regulatory T-cell and T-helper 17 cell differentiation, regulation of the inflammatory response, and the onset of lung cancer (1,2,7,8).

使用例
 
関連製品
12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2729   Normal Rabbit IgG
3900   Rabbit (DA1E) mAb IgG XP® Isotype Control
4486   SimpleChIP® Human α Satellite Repeat Primers
5537   HIF-1β/ARNT (D28F3) XP® Rabbit mAb
6883   SignalFire™ ECL Reagent
7017   6-Tube Magnetic Separation Rack
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
81126   SimpleChIP® Human NFE2L2 Intron 1 Primers
9002   SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
9003   SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
9004   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
9005   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
9006   ChIP-Grade Protein G Magnetic Beads
9007   ChIP-Grade Protein G Agarose Beads
9863   Protein A Agarose Beads
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

AhR (D5S6H) Rabbit mAb

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