CST logo

#8102 SignalSlide® Phospho-EGF Receptor IHC Controls

 
  • SignalSlide® Phospho-EGF Receptor IHC Controls Data Sheet PDF
CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年12月13日15時35分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#8102S1 Pack (5 Slides)28,000
ログインすると国内在庫状況がご確認いただけます。

*  本製品は受注生産品で、有効期限は製造後2ヶ月です。

EGFR 製品一覧

用途
免疫組織染色 (パラフィン)
製品概要
各コントロールスライドにはEGF 未処理と処理済のKYSE 450 細胞がホルマリン固定/パラフィン包埋され、Phospho-EGFR (Tyr1173) タンパク質の免疫染色のコントロールとして使用します。同じ細胞由来の溶解物でウェスタンブロッティングを行い、EGF 処理の効率を確認しています。

To be used with antibodies: 2235, 2237, 3777, 2236, 2234, 4404, 4407, 4267, 9411, 9417, 9416.

貯法
4℃
社内データ

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded KYSE450 cells, untreated (left) or treated with EGF (right), using Phospho-EGF Receptor (Tyr1068) (D7A5) XP (TM) Rabbit mAb #3777.

Schematic

Schematic
バックグラウンド

The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
1110   Phospho-EGF Receptor (Tyr1068) Blocking Peptide
1175   Phospho-EGF Receptor (Tyr1173) Blocking Peptide
13079   Immunohistochemistry Application Solutions Kit (Rabbit)
14166   Hematoxylin
14177   SignalStain® Mounting Medium
14747   SignalStain® EDTA Unmasking Solution (10X)
15019   Animal-Free Blocking Solution (5X)
2234   Phospho-EGF Receptor (Tyr1068) Antibody
2235   Phospho-EGF Receptor (Tyr992) Antibody
2236   Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb
3777   Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb
4267   EGF Receptor (D38B1) XP® Rabbit mAb
4404   Phospho-EGF Receptor (Tyr1148) Antibody
4407   Phospho-EGF Receptor (Tyr1173) (53A5) Rabbit mAb
8059   SignalStain® DAB Substrate Kit
8112   SignalStain® Antibody Diluent
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
8125   SignalStain® Boost IHC Detection Reagent (HRP, Mouse)
8713   Phospho-PLCγ1 (Ser1248) (D25A9) Rabbit mAb
9411   Phospho-Tyrosine Mouse mAb (P-Tyr-100)
9416   Phospho-Tyrosine Mouse mAb (P-Tyr-102)
9417   Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated)
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)

SignalSlide is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

SignalSlide® Phospho-EGF Receptor IHC Controls

Immune Cell Signaling Pathways

お薦めリンク

テクニカルサポート

製品特集

Applications