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#8101 SignalSlide® Phospho-Akt (Ser473) IHC Controls

 
  • SignalSlide® Phospho-Akt (Ser473) IHC Controls Data Sheet PDF
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2020年2月28日15時15分 現在
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#8101S1 Pack (5 Slides)30,000
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*  本製品は受注生産品で、有効期限は製造後2ヶ月です。

AKT1 製品一覧

用途
免疫組織染色 (パラフィン)
製品概要
各コントロールスライドにはPI3-Kinase 阻害剤のLY294002 未処理と処理済のLNCaP 細胞がホルマリン固定/パラフィン包埋され、Phospho-Akt (Ser473) タンパク質の免疫染色のコントロールとして使用します。同細胞由来の溶解物でウェスタンブロッティングを行い、LY294002 処理の効率を確認しております。

To be used with antibodies: 2855, 9644, 4060, 3787, 2938, 4691, 4685, 2920, 9323, 5482, 5196, 2997, 2691, 4858, 4857, 2211, 5364, 2217, 2317.

貯法
4℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from LNCaP cells, untreated or treated with LY294002, using Akt Antibody #9272 (upper) or

Phospho-Akt (Ser 473) (193H121) Rabbit mAb #4058 (lower). This assay serves as a control for the efficacy of the LY94002 treatment.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded LNCaP cells untreated (left) or treated with LY294002 (right) using Phospho-Akt (Ser473) (736E11) Rabbit mAb (IHC Specific) #3787.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded LNCaP cells, untreated (left) or treated with LY294002 (right) using Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060.


Schematic

Schematic
バックグラウンド

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip1 (15) and p21 Waf1/Cip1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).

使用例
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関連製品
1085   Akt (pan) Blocking Peptide
1140   Phospho-Akt (Ser473) Blocking Peptide
1155   S6 Ribosomal Protein Blocking Peptide
11899   Phospho-NDRG1 (Ser330) (D3A12) Rabbit mAb
1220   Phospho-S6 Ribosomal Protein (Ser235/236) Blocking Peptide
13079   Immunohistochemistry Application Solutions Kit (Rabbit)
13175   Phospho-PRAS40 (Thr246) (D4D2) XP® Rabbit mAb
14166   Hematoxylin
14177   SignalStain® Mounting Medium
14746   SignalStain® Citrate Unmasking Solution (10X)
15019   Animal-Free Blocking Solution (5X)
2211   Phospho-S6 Ribosomal Protein (Ser235/236) Antibody
2217   S6 Ribosomal Protein (5G10) Rabbit mAb
2317   S6 Ribosomal Protein (54D2) Mouse mAb
2691   PRAS40 (D23C7) XP® Rabbit mAb
2855   Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb
2920   Akt (pan) (40D4) Mouse mAb
2938   Akt1 (C73H10) Rabbit mAb
2997   Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb
3787   Phospho-Akt (Ser473) (736E11) Rabbit mAb
4060   Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb
4685   Akt (pan) (11E7) Rabbit mAb
4691   Akt (pan) (C67E7) Rabbit mAb
4857   Phospho-S6 Ribosomal Protein (Ser235/236) (91B2) Rabbit mAb
4858   Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb
5196   NDRG1 Antibody
5364   Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb
5482   Phospho-NDRG1 (Thr346) (D98G11) XP® Rabbit mAb
8059   SignalStain® DAB Substrate Kit
8112   SignalStain® Antibody Diluent
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
8125   SignalStain® Boost IHC Detection Reagent (HRP, Mouse)
9315   GSK-3β (27C10) Rabbit mAb
9644   4E-BP1 (53H11) Rabbit mAb
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)

SignalSlide is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

SignalSlide® Phospho-Akt (Ser473) IHC Controls

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