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#7558 HDAC6 (D2E5) Rabbit mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年12月13日15時35分 現在
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#7558S100 μL56,000
#7558T20 μL39,000
Custom Antibody Sampler Kitの構成品を選択できます。

HDAC6 製品一覧 | #7558 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
内在性160Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、サル -
7558 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:100)免疫組織染色 (パラフィン) (1:400)免疫蛍光細胞染色 (IF-IC) (1:200)フローサイトメトリー (1:200)


IHC-P: 抗体希釈液 / 抗原賦活化


内在性レベルのHDAC6 タンパク質を検出します。
ヒトのHDAC6 タンパク質のC末端周辺領域 (組換えタンパク質)

ホモロジー (相同性) 検索をご希望の場合 >>>



※下記の社内データは、すべて7558 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HDAC6 (D2E5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HDAC6 (D2E5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using HDAC6 (D2E5) Rabbit mAb.



Confocal immunofluorescent analysis of A549 cells, untreated (left) or treated with MG132 (5 μM, 24 hr; right), using HDAC6 (D2E5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5®#4084 (fluorescent DNA dye).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of K562 cells using HDAC6 (D2E5) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.


HDAC6 is a class II histone deacetylase enzyme localized to the cytoplasm and associated with the microtubule network (1). It is involved in the regulation of many cellular processes, including cell migration, immune synapse formation, viral infection, and degradation of misfolded proteins (1). HDAC6 contains two tandem catalytic domains that facilitate the deacetylation of multiple protein substrates, including histones and non-histone proteins such as tubulin, cortactin, and HSP90. Despite the ability to deacetylate histone proteins in vitro, there is no evidence for HDAC6-mediated deacetylation of histones in vivo (2,3). The acetylation/deacetylation of tubulin on Lys40 regulates binding and motility of the kinesin-1 motor protein and subsequent transport of cargo proteins such as JNK-interacting protein 1 (JIP1) (4). The acetylation/deacetylation of cortactin regulates cell motility by modulating the binding of cortactin to F-actin (5). Acetylation/deacetylation of HSP90 modulates chaperone complex activity by regulating the binding of an essential cochaperone protein, p23 (6,7). In addition to its role as a protein deacetylase, HDAC6 functions as a component of the aggresome, a proteinaceous inclusion body that forms in response to an accumulation of misfolded or partially denatured proteins (8). Formation of the aggresome is a protective response that sequesters cytotoxic protein aggregates for eventual autophagic clearance from the cell. HDAC6 contains a zinc finger ubiquitin-binding domain that binds both mono- and poly-ubiquitinated proteins (8). HDAC6 binds to both poly-ubiquitinated misfolded proteins and dynein motors, facilitating the transport of misfolded proteins to the aggresome (9,10). HDAC6 is also required for subsequent recruitment of the autophagic machinery and clearance of aggresomes from the cell (11). Thus, HDAC6 plays a key role in the protection against the deleterious effects of pathological protein aggregation that occurs in various diseases, such as neurodegenerative Huntington’s disease (11).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2125   α-Tubulin (11H10) Rabbit mAb
2128   β-Tubulin (9F3) Rabbit mAb
3502   Cortactin Antibody
3503   Cortactin (H222) Antibody
4356   HSF1 Antibody
4877   HSP90 (C45G5) Rabbit mAb
5335   Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8112   SignalStain® Antibody Diluent
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

DRAQ5 is a registered trademark of Biostatus Limited.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


HDAC6 (D2E5) Rabbit mAb

Immune Cell Signaling Pathways