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#7003 20X LumiGLO® Reagent and 20X Peroxide

  • 20X LumiGLO® Reagent and 20X Peroxide Data Sheet PDF
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希望納入価格 (円)
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2019年12月6日15時40分 現在
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#7003P5 mL each14,000
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#7003S1 Pack36,000
内容容量
20X LumiGLO® (luminol chemiluminescent substrate)
20X Peroxide
25 mL
25 mL
製品概要
HRP 標識二次抗体を用いたウェスタンブロッティングの検出に用いる試薬です。非常に高感度に検出することが可能です。
貯法
4℃
社内データ

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

バックグラウンド

Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.

使用文献
 
関連製品
7074   Anti-rabbit IgG, HRP-linked Antibody
7075   Anti-biotin, HRP-linked Antibody
7076   Anti-mouse IgG, HRP-linked Antibody
7077   Anti-rat IgG, HRP-linked Antibody

LumiGLO is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

20X LumiGLO® Reagent and 20X Peroxide

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