#6888 Phospho-ULK1 (Ser757) Antibody
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CSTコード | 包装 | 希望納入価格 (円) |
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#6888S | 100 μL | 66,000 | ログインすると国内在庫状況がご確認いただけます。
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#6888T | 20 μL | 39,000![]() Custom Antibody Sampler Kitの構成品を選択できます。 5本以上を選択し、ページ右上のCartから製品確定書を発行してください。 尚、構成品の単品販売は致しておりません。 |
ULK1 製品一覧 | #6888 が入っているAntibody Sampler キット一覧
感度 | 分子量 (kDa) | 抗体の由来 | 貯法 | |
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内在性 | 140-150 | Rabbit | -20℃ |
種交差性 (社内試験済) | |
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ヒト、マウス、サル | - |
特異性・感度 | |
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内在性レベルのSer757 がリン酸化されたマウスのULK1 タンパク質 (ヒトのULK1 タンパク質のSer758 に相当) を検出します。 |
使用抗原 | |
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マウスのULK1 タンパク質のSer757 (ヒトのULK1 タンパク質のSer758 に相当) 周辺領域 (合成リン酸化ペプチド) |
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社内データ |
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※下記の社内データは、すべて6888 の推奨プロトコールで実験した結果です。
Western Blotting

Western blot analysis of extracts from A172 cells, untreated (-), Torin 1-treated (+; 250 nM; 5 hrs), or INK-128-treated (+; 250 nM; 5 hrs) using Phospho-ULK1 (Ser757) Antibody (upper) or total ULK1 (D8H5) Rabbit mAb #8054 (lower).
バックグラウンド |
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Two related serine/threonine kinases, UNC-51-like kinase 1 and 2 (ULK1, ULK2), were discovered as mammalian homologs of the C. elegans gene UNC-51 in which mutants exhibited abnormal axonal extension and growth (1-4). Both proteins are widely expressed and contain an amino-terminal kinase domain followed by a central proline/serine rich domain and a highly conserved carboxy-terminal domain. The roles of ULK1 and ULK2 in axon growth have been linked to studies showing that the kinases are localized to neuronal growth cones and are involved in endocytosis of critical growth factors, such as NGF (5). Yeast two-hybrid studies found ULK1/2 associated with modulators of the endocytic pathway, SynGAP and syntenin (6). Structural similarity of ULK1/2 has also been recognized with the yeast autophagy protein Atg1/Apg1 (7). Knockdown experiments using siRNA demonstrated that ULK1 is essential for autophagy (8), a catabolic process for the degradation of bulk cytoplasmic contents (9,10). It appears that Atg1/ULK1 can act as a convergence point for multiple signals that control autophagy (11), and can bind to several autophagy-related (Atg) proteins, regulating phosphorylation states and protein trafficking (12-16).
AMPK, activated during low nutrient conditions, directly phophorylates ULK1 at multiple sites including Ser317, Ser555, and Ser777 (17, 18). Conversely, mTOR, which is a regulator of cell growth and is an inhibitor of autophagy, phosphorylates ULK1 at Ser757 and disrupts the interaction between ULK1 and AMPK (17).
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- Kuroyanagi, H. et al. (1998) Genomics 51, 76-85.
- Yan, J. et al. (1998) Biochem Biophys Res Commun 246, 222-7.
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- Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
- Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.
- Stephan, J.S. and Herman, P.K. (2006) Autophagy 2, 146-8.
- Okazaki, N. et al. (2000) Brain Res Mol Brain Res 85, 1-12.
- Young, A.R. et al. (2006) J Cell Sci 119, 3888-900.
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使用例 |
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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
本製品は試験研究用です。