CST logo

#68851 G9a/EHMT2 (D5R4R) XP® Rabbit mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年11月12日15時40分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#68851S100 μL61,000
ログインすると国内在庫状況がご確認いただけます。
#68851T20 μL39,000
Custom Antibody Sampler Kitの構成品を選択できます。
5本以上を選択し、ページ右上のCartから製品確定書を発行してください。
尚、構成品の単品販売は致しておりません。

G9A 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性160, 180Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト、マウス、ラット、サル -
68851 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:1000)免疫沈降 (1:100)免疫蛍光細胞染色 (IF-IC) (1:3200)クロマチン免疫沈降 (1:50)

CST推奨プロトコールに欠かせない関連製品

特異性・感度
内在性レベルのG9a/EHMT2 タンパク質を検出します。
使用抗原
ヒトのG9a/EHMT2 タンパク質のN末端領域 (組換えタンパク質)

ホモロジー (相同性) 検索をご希望の場合 >>>

ホモロジー検索をご要望の際は、ご希望のサンプル種のアミノ酸配列とともにお問合せください。

社内データ

※下記の社内データは、すべて68851 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using G9a/EHMT2 (D5R4R) XP® Rabbit mAb.

IP

IP

Immunoprecipitation of G9a from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is G9a/EHMT2 (D5R4R) XP® Rabbit mAb. Western blot analysis was performed using G9a/EHMT2 (D5R4R) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using G9a/EHMT2 (D5R4R) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).


Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from K-562 cells and either G9a/EHMT2 (D5R4R) XP® Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR using human VRK3 intron 11 primers, SimpleChIP® Human ZNF19 Intron 3 Primers #98428, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

バックグラウンド

G9a, also known as Euchromatic histone-lysine N-methyltransferase 2 (EHMT2), is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1). Recombinant G9a can mono-, di- and tri-methylate histone H3 on Lys9 and Lys27 in vitro (1,2). However, in vivo G9a forms a complex with GLP, a G9a-related histone methyltransferase, and together these proteins function as the major euchromatic histone H3 Lys9 mono- and di-methyltransferases, creating transcriptionally repressive marks that facilitate gene silencing (3,4). G9a methylates itself on Lys165, a modification that regulates the association of HP1 repressor proteins with the G9a/GLP complex (5). The G9a/GLP complex also contains Wiz, a zinc finger protein that is required for G9a/GLP hetero-dimerization and complex stability (6). Wiz contains two CtBP co-repressor binding sites, which mediate the association of the G9a/GLP with the CtBP co-repressor complex (6). In addition, G9a and GLP are components of other large transcriptional co-repressor complexes, such as those involving E2F6 and CDP/cut (7-9). G9a interacts with DNMT1, and both proteins are required for methylation of DNA and histone H3 (Lys9) at replication foci, providing a functional link between histone H3 Lys9 and CpG methylation during DNA replication (10). G9a activity is critical for meiotic prophase progression, as mutant mice deficient in germ line G9a show a large loss of mature gametes (11). In addition, G9a facilitates increased global levels of di-methyl histone H3 (Lys9) during hypoxic stress and increased G9a expression is associated with hepatocelluar carcinoma (12,13).

使用例
 
関連製品
13969   Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb
14186   Mono-Methyl-Histone H3 (Lys9) (D1P5R) Rabbit mAb
14654   12-Tube Magnetic Separation Rack
2613   HP1β Antibody
2616   HP1α Antibody
2619   HP1γ Antibody
2623   HP1α/β (C7F11) Rabbit mAb
3306   G9a/EHMT2 (C6H3) Rabbit mAb
4486   SimpleChIP® Human α Satellite Repeat Primers
4499   Histone H3 (D1H2) XP® Rabbit mAb
4620   Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated)
4658   Di-Methyl-Histone H3 (Lys9) (D85B4) XP® Rabbit mAb
56383   SimpleChIP® Plus Sonication Chromatin IP Kit
7003   20X LumiGLO® Reagent and 20X Peroxide
7017   6-Tube Magnetic Separation Rack
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8676   HP1β (D2F2) XP® Rabbit mAb
9002   SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
9003   SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
9004   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)
9005   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
9006   ChIP-Grade Protein G Magnetic Beads
9007   ChIP-Grade Protein G Agarose Beads
98428   SimpleChIP® Human ZNF19 Intron 3 Primers
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

本製品は試験研究用です。

G9a/EHMT2 (D5R4R) XP® Rabbit mAb

Immune Cell Signaling Pathways

お薦めリンク

テクニカルサポート

製品特集

Applications