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#6436 SignalSilence® p44 MAPK (Erk1) siRNA I

 
  • SignalSilence® p44 MAPK (Erk1) siRNA I Data Sheet PDF
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2020年2月17日11時40分 現在
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ERK1 製品一覧

種交差性機能すると推定される種
ヒトマウス、ラット、サル
特異性
p44 MAPK (Erk1) タンパク質の発現を特異的に抑制します。
貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® p44 MAPK (Erk1) siRNA I (+), using p44/42 MAPK (Erk1/2) Antibody #9102 and GCK Antibody #3782. The Erk1/2 antibody confirms silencing of Erk1 expression and GCK antibody is used to control for loading and specificity of p44 MAPK (Erk1) siRNA.

Fluorescent Detection

Fluorescent Detection

Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency.

バックグラウンド

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors, such as U0126 and PD98059.

Small interfering RNA (siRNA) has been used to specifically silence p44 MAPK (Erk1) expression in HEK293 cells (7).

使用例
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関連製品
3782   GCK Antibody
6426   SignalSilence® MEK1 siRNA I
6431   SignalSilence® MEK2 siRNA I
9102   p44/42 MAPK (Erk1/2) Antibody

SignalSilence is a registered trademark of Cell Signaling Technology, Inc.
Limited Use Label License, RNA interference: This product is licensed under European Patent 1144623 and foreign equivalents from Ribopharma AG, Kulmbach, Germany and is provided only for use in non-commercial research specifically excluding use (a) in drug discovery or drug development, including target identification or target validation, by or on behalf of a commercial entity, (b) for contract research or commercial screening services, (c) for the production or manufacture of siRNA-related products for sale, or (d) for the generation of commercial databases for sale to Third Parties. Information about licenses for these and other commercial uses is available from Ribopharma AG, Fritz-Hornschuch-Str. 9, D-95326 Kulmbach, Germany.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

SignalSilence® p44 MAPK (Erk1) siRNA I

Immune Cell Signaling Pathways

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