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#6301 SignalSilence® GSK-3α/β siRNA

  • SignalSilence® GSK-3α/β siRNA Data Sheet PDF
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希望納入価格 (円)
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2019年12月9日11時40分 現在
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#6301S300 μL55,000
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GSK3 製品一覧

種交差性機能すると推定される種
ヒトマウス、ラット
特異性
GSK-3αおよびGSK-3βタンパク質の発現を特異的に抑制します。
貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untransfected or transfected with GSK-3a/β siRNA. GSK-3β was detected using GSK-3β Antibody #9332, and Bcl-xL was detected using Bcl-xL Antibody #2762. The GSK-3β Antibody confirms silencing of GSK-3β expression, and the Bcl-xL Antibody is used to control for loading and siRNA specificity.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untransfected or transfected with either GSK-3alpha/beta siRNA or GSK-3alpha siRNA. GSK-3alpha and GSK-3beta were detected using a GSK-3alpha/beta antibody, and eIF4B was detected using eIF4B Antibody #3592. The GSK-3alpha/beta antibody confirms silencing of GSK-3alpha and beta expression, and the eIF4B Antibody is used to control for loading and siRNA specificity.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, mock-transfected (left) or transfected with SignalSilence® GSK-3α/β siRNA (right), using GSK-3-alpha/beta (D75D3) XP® Rabbit mAb #5676 (green). Actin filaments have been labeled wth DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


バックグラウンド

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

Small Interfering RNA (siRNA) has been used to specifically silence GSK-3alpha and beta expression, causing an upregulation of beta-catenin protein levels (6).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
6201   SignalSilence® Control siRNA (Fluorescein Conjugate)
6568   SignalSilence® Control siRNA (Unconjugated)
9315   GSK-3β (27C10) Rabbit mAb
9331   Phospho-GSK-3α/β (Ser21/9) Antibody

DRAQ5 is a registered trademark of Biostatus Limited.
SignalSilence is a registered trademark of Cell Signaling Technology, Inc.
Limited Use Label License, RNA interference: This product is licensed under European Patent 1144623 and foreign equivalents from Ribopharma AG, Kulmbach, Germany and is provided only for use in non-commercial research specifically excluding use (a) in drug discovery or drug development, including target identification or target validation, by or on behalf of a commercial entity, (b) for contract research or commercial screening services, (c) for the production or manufacture of siRNA-related products for sale, or (d) for the generation of commercial databases for sale to Third Parties. Information about licenses for these and other commercial uses is available from Ribopharma AG, Fritz-Hornschuch-Str. 9, D-95326 Kulmbach, Germany.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

SignalSilence® GSK-3α/β siRNA

Metabolic Reprogramming in Disease

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