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#6269 SignalSilence® p38α MAPK siRNA I

  • SignalSilence® p38α MAPK siRNA I Data Sheet PDF
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2020年2月17日11時40分 現在
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MAPKP38-ALPHA 製品一覧

種交差性機能すると推定される種
ヒト-
特異性
p38α MAPK タンパク質の発現を特異的に抑制します。
貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® p38α MAPK siRNA I (+) or SignalSilence® p38α MAPK siRNA II #6277 (+), using p38α MAPK Antibody #9218 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The p38α MAPK Antibody confirms silencing of p38α MAPK expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

バックグラウンド

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).

使用例
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関連製品
2325   Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb
6201   SignalSilence® Control siRNA (Fluorescein Conjugate)
6277   SignalSilence® p38α MAPK siRNA II
6568   SignalSilence® Control siRNA (Unconjugated)
9218   p38α MAPK Antibody

SignalSilence is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

SignalSilence® p38α MAPK siRNA I

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