#5934 Okadaic Acid
|セリン/スレオニンタンパク質ホスファターゼであるPP1 とPP2A を阻害します。|
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Okadaic Acid (1 µM, 1 hr; +), using Phospho-LATS1 (Thr1079) (D57D3) Rabbit mAb #8654 (upper) or LATS1 (C66B5) Rabbit mAb #3477 (lower).
Western blot analysis of extracts from HeLa cells, untreated or treated with Okadaic Acid (1 hr) at the indicated concentrations, using Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Okadaic acid is an inhibitor of the serine/theorine protein phosphatases PP1 (IC50 = 15-20 nM) and PP2A (IC50 = 0.1 nM), exhibiting much greater activity towards the latter. PP2A is completely inhibited at 1-2 nM, compared to greater than 1 µM for PP1 (1). This is unlike the protein phosphatase inhibitor Calyculin A, which potently inhibits both PP1 (IC50 = 2 nM) and PP2A (IC50 = 0.5-1 nM) (2). Okadaic acid does not effectively inhibit PP2C (3), acid and alkaline phosphatases, nor phosphotyrosine protein phosphatases (2,3). It has shown to inhibit PP2B, but at much higher concentrations than with PP1 and PP2A (3). Okadaic acid has also been described as a non-TPA type potent tumor promoter (4). These tumor-promoting properties and its ability to significantly increase protein phosphorylation make okadaic acid a very useful tool for studying cellular processes that are regulated by reversible phosphorylation of proteins (5).
|12611 DMSO (Dimethyl Sulfoxide), Sterile|
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