CST logo

#5934 Okadaic Acid

  • Okadaic Acid Data Sheet PDF
CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年8月23日15時25分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#5934S25 μg22,000
ログインすると国内在庫状況がご確認いただけます。
概要
セリン/スレオニンタンパク質ホスファターゼであるPP1 とPP2A を阻害します。
分子量 
805.00
分子の化学式
C44H68O13
貯法
-20℃
社内データ

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Okadaic Acid (1 µM, 1 hr; +), using Phospho-LATS1 (Thr1079) (D57D3) Rabbit mAb #8654 (upper) or LATS1 (C66B5) Rabbit mAb #3477 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with Okadaic Acid (1 hr) at the indicated concentrations, using Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Structure

Structure

Chemical structure of okadaic acid.


バックグラウンド

Okadaic acid is an inhibitor of the serine/theorine protein phosphatases PP1 (IC50 = 15-20 nM) and PP2A (IC50 = 0.1 nM), exhibiting much greater activity towards the latter. PP2A is completely inhibited at 1-2 nM, compared to greater than 1 µM for PP1 (1). This is unlike the protein phosphatase inhibitor Calyculin A, which potently inhibits both PP1 (IC50 = 2 nM) and PP2A (IC50 = 0.5-1 nM) (2). Okadaic acid does not effectively inhibit PP2C (3), acid and alkaline phosphatases, nor phosphotyrosine protein phosphatases (2,3). It has shown to inhibit PP2B, but at much higher concentrations than with PP1 and PP2A (3). Okadaic acid has also been described as a non-TPA type potent tumor promoter (4). These tumor-promoting properties and its ability to significantly increase protein phosphorylation make okadaic acid a very useful tool for studying cellular processes that are regulated by reversible phosphorylation of proteins (5).

使用例
製品をご使用いただいて研究を発表されましたら、ぜひお知らせください
 
関連製品
12611   DMSO (Dimethyl Sulfoxide), Sterile

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Okadaic Acid

Metabolic Reprogramming in Disease

お薦めリンク

テクニカルサポート

製品特集

Applications