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#58295 Akt (pan) (E7J2C) Mouse mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年11月21日15時20分 現在
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#58295S100 μL56,000

AKT 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性60Mouse IgG2a-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス、ラット、サル -
58295 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:50)免疫蛍光細胞染色 (IF-IC) (1:200)フローサイトメトリー (1:200-1:800)


Akt (pan) (E7J2C) Mouse mAb recognizes endogenous levels of total Akt protein.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Akt.

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※下記の社内データは、すべて58295 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of various cell lines and recombinant Akt1, Akt2 and Akt3 proteins using Akt (pan) (E7J2C) Mouse mAb. Recombinant Akt1, Akt2 and Akt3 each contain an N-terminal 6XHis and FLAG-Tag, MW=66kDa.

Western Blotting

Western Blotting

Western blot analysis of C2C12 cell extracts using Akt (pan) (E7J2C) Mouse mAb as the primary antibody. Various anti-mouse isotype specific antibodies (upper) and Anti-mouse IgG, HRP-linked Antibody #7076 (lower) were used as secondary antibodies.



Immunoprecipitation of Akt protein from Jurkat cell extracts. Lane 1 is 10% input, lane 2 is Mouse (E5Y6Q) mAb IgG2a Isotype Control #61656, and lane 3 is Akt (pan) (E7J2C) Mouse mAb. Western blot analysis was performed using Akt (pan) (E7J2C) Mouse mAb.



Confocal immunofluorescent analysis of C2C12 cells, treated with LY294002 #9901 (50 μM, 2 hr; left) or insulin (100 nM, 20 min; right), using Akt (pan) (E7J2C) Mouse mAb (green). Blue = DAPI #4083 (fluorescent DNA dye). Note the translocation of Akt to ruffling membranes following stimulation.



Confocal immunofluorescent analysis of A549 cells, treated with insulin (100 nM, 20 min; top) or LY294002 #9901 (50 μM, 2 hr; bottom) using Akt (pan) (E712C) Mouse mAB (green; mouse IgG2a), α-Tubulin (DM1A) Mouse mAb #3873 (yellow;mouse IgG1), and Phospho-S6 Ribosomal Protein (Ser240/244) (D68F8) XP® Rabbit mAb #5364 (red; rabbit IgG). Samples were mounted in Prolong Gold Antifade Reagent® with DAPI #8961 (blue). Note the translocation of Akt to ruffling membranes following stimulation with insulin (arrow heads).


Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip1 (15) and p21 Waf1/Cip1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
13953   Prestained Protein Marker, Broad Range (11-190 kDa)
2920   Akt (pan) (40D4) Mouse mAb
2966   Akt (5G3) Mouse mAb
37478   Protein G Agarose Beads
4685   Akt (pan) (11E7) Rabbit mAb
4691   Akt (pan) (C67E7) Rabbit mAb
6883   SignalFire™ ECL Reagent
7003   20X LumiGLO® Reagent and 20X Peroxide
7075   Anti-biotin, HRP-linked Antibody
7076   Anti-mouse IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9272   Akt Antibody
9774   Loading Control Antibody Sampler Kit (Mouse)
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

Tween is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.


Akt (pan) (E7J2C) Mouse mAb

Metabolic Reprogramming in Disease