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#56795 SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina®

 
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希望納入価格 (円)
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2019年9月13日15時30分 現在
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キット内容 容量 Storage Temp
End Prep Enzyme Mix 1 x 72 µl -20°C
End Prep Reaction Buffer 1 x 168 µl -20°C
Ligation Enhancer 1 x 24 µl -20°C
Ligation Master Mix 1 x 720 µl -20°C
Q5® PCR Master Mix 1 x 600 µl -20°C
貯法
-20℃
社内データ

Chromatin IP-seq

Chromatin IP-seq

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT 116 cells and Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared from 50 ng, 5 ng, or 0.5 ng enriched ChIP DNA or 50 ng Input DNA using SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Dual Index Primers) #47538, pooled into one sample, and sequenced on an Illumina® Next-Seq platform. The figure shows binding across GAPDH, a known target gene of H3K4me3. For additional ChIP-seq tracks, please download the product data sheet.

Chromatin IP-seq

Chromatin IP-seq

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT 116 cells and TCF4/TCF7L2 (C48H11) Rabbit mAb #2569, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Libraries were prepared from 50 ng, 5 ng, or 0.5 ng enriched ChIP DNA or 50 ng Input DNA using SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Dual Index Primers) #47538, pooled into one sample, and sequenced on an Illumina® Next-Seq platform. The figure shows binding across CaMK2D, a known target gene of TCF4/TCF7L2. For additional ChIP-seq tracks, please download the product data sheet.

Chromatin IP-seq

Chromatin IP-seq

Metagene analysis of ChIP-seq data generated using different amounts of starting ChIP DNA. Analyses of both Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751 (left) and TCF4/TCF7L2 (C48H11) Rabbit mAb #2569 (right) ChIP-seq data show that the signal-to-noise ratio for peaks identified across entire genome are similar for all three starting amounts of ChIP DNA.


バックグラウンド

The chromatin immunoprecipitation (ChIP) assay is a powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell (1,2). This assay can be used to identify multiple proteins associated with a specific region of the genome, or the opposite, to identify the many regions of the genome bound by a particular protein (3-6). It can be used to determine the specific order of recruitment of various proteins to a gene promoter or to "measure" the relative amount of a particular histone modification across an entire gene locus (3,4). In addition to histone proteins, the ChIP assay can be used to analyze binding of transcription factors and co-factors, DNA replication factors and DNA repair proteins. When performing the ChIP assay, cells or tissues are first fixed with formaldehyde, a reversible protein-DNA cross-linking agent that "preserves" the protein-DNA interactions occurring in the cell (1,2). Cells are lysed and chromatin is harvested and fragmented using either sonication or enzymatic digestion. The chromatin is then immunoprecipitated with antibodies specific to a particular protein or histone modification. Any DNA sequences that are associated with the protein or histone modification of interest will co-precipitate as part of the cross-linked chromatin complex and the relative amount of that DNA sequence will be enriched by the immunoselection process. After immunoprecipitation, the protein-DNA cross-links are reversed and the DNA is purified. Standard PCR or Quantitative Real-Time PCR can be used to measure the amount of enrichment of a particular DNA sequence by a protein-specific immunoprecipitation (1,2). Alternatively, the ChIP assay can be combined with genomic tiling micro-array (ChIP on chip) techniques, high throughput sequencing, or cloning strategies, all of which allow for genome-wide analysis of protein-DNA interactions and histone modifications (5-8).

使用例
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関連製品
14209   SimpleChIP® DNA Purification Buffers and Spin Columns
14654   12-Tube Magnetic Separation Rack
29580   SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Single Index Primers)
47538   SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Dual Index Primers)
56383   SimpleChIP® Plus Sonication Chromatin IP Kit
7017   6-Tube Magnetic Separation Rack
88989   SimpleChIP® Universal qPCR Master Mix
9003   SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
9005   SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)
9006   ChIP-Grade Protein G Magnetic Beads

Illumina is a registered trademark of Illumina, Inc.
AMPure is a registered trademark of Beckman Coulter, Inc.
Q5 is a registered trademark of New England Biolabs, Inc.
SPRIselect is a registered trademark of Beckman Coulter, Inc.
Bioanalyzer is a registered trademark of Agilent Technologies, Inc
USER Enzyme is a registered trademark of New England Biolabs, Inc.
SimpleChIP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina®

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