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#5634 EGF Receptor Control Cell Extracts

  • EGF Receptor Control Cell Extracts Data Sheet PDF
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希望納入価格 (円)
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2019年12月6日15時40分 現在
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CELL EXTRACTS 製品一覧

調製方法
Nonphosphorylated EGF Receptor Control Cell Extracts: 血清飢餓状態で一晩培養したA431 細胞の溶解物
Phosphorylated EGF Receptor Control Cell Extracts: hEGF #8916 で処理 (100 ng/mL、5分間) したA431 細胞の溶解物
貯法
-20℃
MSDSはこちらから
社内データ

Western Blotting

Western Blotting

Western blot analysis of EGF Receptor Control Cell extracts using Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb #3777 (upper) and EGF Receptor (D38B1) XP® Rabbit mAb #4267 (lower).

バックグラウンド

The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation (1,2). Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins (3,4). c-Src is involved in phosphorylation of EGFR at Tyr845 (5). The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling (6). Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation (7,8). The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 (9). A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation (2). Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation (10).

使用例
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2235   Phospho-EGF Receptor (Tyr992) Antibody
2236   Phospho-EGF Receptor (Tyr1068) (1H12) Mouse mAb
2237   Phospho-EGF Receptor (Tyr1045) Antibody
2238   Phospho-EGF Receptor (Ser1046/1047) Antibody
2239   EGF Receptor (1F4) Mouse mAb
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2641   Phospho-EGF Receptor (Tyr998) (C24A5) Rabbit mAb
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3265   EGF Receptor (V279) Antibody
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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

EGF Receptor Control Cell Extracts

Immune Cell Signaling Pathways

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