#5545 hnRNP A0 (D8A3) XP^® Rabbit mAb

Western Blotting

Western blot analysis of extracts from various cell lines using hnRNP A0 (D8A3) XP^® Rabbit mAb.

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with SignalSilence^® Control siRNA (Unconjugated) #6568 (-) or SignalSilence^® hnRNP A0 siRNA I #6503 (+), using hnRNP A0 (D8A3) XP^® Rabbit mAb (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower).

IF-IC

Confocal immunofluorescent analysis of HeLa cells using hnRNP A0 (D8A3) XP^® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Flow Cytometry

Flow cytometric analysis of HeLa cells using hnRNP A0 (D8A3) XP^® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

Heterogeneous nuclear ribonucleoprotein A0 (hnRNP A0) is a member of the hnRNP A/B family of related RNA binding proteins that bind pre-mRNA and are involved in the processing, metabolism, and transport of nuclear pre-mRNA transcripts (1). The A/B subfamily of hnRNP includes A1, A2/B1, A3, and A0. hnRNP A0 is phosphorylated at Ser84 by MAPKAPK-2 in response to LPS treatment in mouse macrophage cells, which might play a key role in stimulating translation of the TNF-α message (2).

1. Myer, V.E. and Steitz, J.A. (1995) RNA 1, 171-82.
2. Rousseau, S. et al. (2002) EMBO J 21, 6505-14.