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#5415 Mouse (G3A1) mAb IgG1 Isotype Control

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年11月15日15時30分 現在
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#5415S250 μg25,000
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感度抗体の由来貯法
内在性Mouse IgG1, kappa-20℃
5415 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

免疫沈降 (-)クロマチン免疫沈降 (-)

CST推奨プロトコールに欠かせない関連製品

濃度
2.5 mg/mL
社内データ

※下記の社内データは、すべて5415 の推奨プロトコールで実験した結果です。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human seminoma using Nanog (1E6C4) Mouse mAb #4893 (left) or Mouse (G3A1) mAb IgG1 Isotype Control (right).

IF-IC

IF-IC

Confocal immunofluorescent analysis of normal rat cerebellum using Neurofilament H (RMdO 20) Mouse mAb #2836 (green, left) compared to concentration matched Mouse (G3A1) mAb IgG1 Isotype Control (green, right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HT-29 cells using α-Tubulin (DM1A) Mouse mAb #3873 (green, left) compared to concentration matched Mouse (G3A1) mAb IgG1 Isotype Control (green, right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of MCF7 cells using Pan-Keratin (C11) Mouse mAb #4545 (blue) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control (red).

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, U0126-treated (blue) or TPA-treated (green), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb #9106 compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control (red).

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed using digested chromatin from HeLa cells and the indicated antibodies. Purified DNA was analyzed by quantitative real-time PCR, using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, and SimpleChIP® Human MYT-1 Exon 1 Primers #4493. The relative abundance of each DNA sequence enriched by Mouse (G3A1) mAb IgG1 Isotype Control (red) is compared to the amount of the same DNA sequence enriched by the histone H3-specific immunoprecipitations (blue).


バックグラウンド

Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

使用例
 
関連製品
2975   Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate)
2985   Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 647 Conjugate)
3452   Rabbit IgG Isotype Control (Alexa Fluor® 647 Conjugate)
4340   Rabbit IgG Isotype Control (Alexa Fluor® 488 Conjugate)
8112   SignalStain® Antibody Diluent
8125   SignalStain® Boost IHC Detection Reagent (HRP, Mouse)
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)

DRAQ5 is a registered trademark of Biostatus Limited.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Mouse (G3A1) mAb IgG1 Isotype Control

Immune Cell Signaling Pathways

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