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#5355 Phospho-NMDA Receptor 2B (GluN2B) (Ser1284) Antibody

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2020年1月17日15時20分 現在
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#5355S100 μL67,000

NMDAR2B 製品一覧

感度分子量 (kDa)抗体の由来貯法
種交差性 (社内試験済)
交差する可能性がある種 i


マウス、ラット ヒト
5355 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:50)


内在性レベルのSer1284 がリン酸化されたNMDA Recpetor 2B (GluN2B) タンパク質を検出します。
ヒトのNMDA Recpetor 2B (GluN2B) タンパク質のSer1284 周辺領域 (合成リン酸化ペプチド)

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※下記の社内データは、すべて5355 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from rat brain using Phospho-NMDA Receptor 2B (GluN2B) (Ser1284) Antibody. The phospho-specificity of the antibody was verified by blocking with a phospho or non-phosphopeptide.


N-methyl-D-aspartate receptor (NMDAR) forms a heterodimer of at least one NR1 and one NR2A-D subunit. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits. NR1 subunits bind the co-agonist glycine and NR2 subunits bind the neurotransmitter glutamate. Activation of the NMDA receptor or opening of the ion channel allows flow of Na+ and Ca2+ ions into the cell, and K+ out of the cell (1). Each subunit has a cytoplasmic domain that can be directly modified by the protein kinase/phosphatase (2). PKC can phosphorylate the NR1 subunit (NMDAR1) of the receptor at Ser890/Ser896, and PKA can phosphorylate NR1 at Ser897 (3). The phosphorylation of NR1 by PKC decreases its affinity for calmodulin, thus preventing the inhibitory effect of calmodulin on NMDAR (4). The phosphorylation of NR1 by PKA probably counteracts the inhibitory effect of calcineurin on the receptor (5). NMDAR mediates long-term potentiation and slow postsynaptic excitation, which play central roles in learning, neurodevelopment, and neuroplasticity (6).

Ephrin-B2 binding to its receptor EphB leads to the activation of Src family tyrosine kinases, which phosphorylate NMDAR2B at Tyr1252, Tyr1336, and Tyr1472. In turn, phosphorylated NMDAR2B enhances the ability of the functional NMDA receptor to regulate Ca2+ influx in response to glutamate (7). Phosphorylation of NMDAR2B at Ser1284 was identified at Cell Signaling Technology (CST) using PhosphoScan®, an MS/MS platform for phosphorylation site discovery. Phosphorylation of NMDAR2B at Ser1284 was observed in extracts isolated from embryo and adult mouse brain. For additional information please visit PhosphoSitePlus®, a CST modification site knowledgebase, at www.phosphosite.org.

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12757   SignalFire™ Elite ECL Reagent
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4205   NMDA Receptor 2A (GluN2A) Antibody
4206   Phospho-NMDA Receptor 2A (GluN2A) (Tyr1246) Antibody
4207   NMDA Receptor 2B (GluN2B) Antibody
4208   Phospho-NMDA Receptor 2B (GluN2B) (Tyr1472) Antibody
4209   Phospho-NMDA Receptor 2B (GluN2B) (Tyr1070) Antibody
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6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7075   Anti-biotin, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
9997   Tris Buffered Saline with Tween® 20 (TBST-10X)
9999   Nonfat Dry Milk

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Phospho-NMDA Receptor 2B (GluN2B) (Ser1284) Antibody

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