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#5327 Di/Tri-Methyl-Histone H3 (Lys9) (6F12) Mouse mAb

 
CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年8月20日15時55分 現在
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#5327S100 μL66,000
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#5327T20 μL39,000
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HISTONE H3 製品一覧

感度分子量 (kDa)抗体の由来貯法
内在性17Mouse IgG1-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト、マウス、ラット、サル キイロショウジョウバエ、ゼブラフィッシュ、出芽酵母
5327 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:2000)免疫沈降 (1:100)免疫蛍光細胞染色 (IF-IC) (1:100)クロマチン免疫沈降 (1:100)

CST推奨プロトコールに欠かせない関連製品

特異性・感度
内在性レベルのLys9 がジメチル化あるいはトリメチル化されたHistone H3 タンパク質を検出します。Lys9 がモノメチル化されたHistone H3 タンパク質とも若干交差します。Lys4、Lys27、Lys36、Lys79 がメチル化されたHistone H3 タンパク質とは交差しません。
使用抗原
Lys9 がトリメチル化されたHistone H3 タンパク質のN末端近傍領域 (合成ペプチド)

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社内データ

※下記の社内データは、すべて5327 の推奨プロトコールで実験した結果です。

ELISA-Peptide

ELISA-Peptide

Di/Tri-Methyl Histone H3 (Lys9) (6F12) Mouse mAb specificity was determined by peptide ELISA. The graph depicts the binding of the antibody to pre-coated tri-methyl histone H3 (Lys9) peptide in the presence of increasing concentrations of various competitor peptides. As shown, only the di-methyl and tri-methyl histone H3 (Lys9) peptides compete away binding of the antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and NIH/3T3 cell lines using Di/Tri-Methyl-Histone H3 (Lys9) (6F12) Mouse mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Di/Tri-Methyl-Histone H3 (Lys9) (6F12) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).


Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Di/Tri-Methyl-Histone H3 (Lys9) (6F12) Mouse mAb or Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human α Satellite Repeat Primers #4486, and SimpleChIP® Human AFM Intron 1 Primers #5098. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

バックグラウンド

The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker (9).

使用例
 
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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Di/Tri-Methyl-Histone H3 (Lys9) (6F12) Mouse mAb

Immune Cell Signaling Pathways

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