CST logo

#4904 Stat3 (79D7) Rabbit mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年12月13日15時35分 現在
ご登録代理店情報 i
#4904S100 μL56,000
#4904T20 μL39,000
Custom Antibody Sampler Kitの構成品を選択できます。

STAT3 製品一覧 | Stat3 抗体比較

感度分子量 (kDa)抗体の由来貯法
内在性79, 86Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス、ラット、サル -
4904 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:2000)免疫沈降 (1:100)クロマチン免疫沈降 (1:50)


内在性レベルのStat3 タンパク質を検出します。
マウスのStat3 タンパク質のC末端領域 (融合タンパク質)

ホモロジー (相同性) 検索をご希望の場合 >>>



※下記の社内データは、すべて4904 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Stat3 (79D7) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® Stat3 siRNA I (+) or SignalSilence® Stat3 siRNA II #6582 (+), using Stat3 (79D7) Rabbit mAb #4904 and α-Tubulin (11H10) Rabbit mAb #2125. The Stat3 (79D7) Rabbit mAb confirms silencing of Stat3 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Stat3 siRNA.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from Hep G2 cells treated with IL-6 (100 ng/ml) for 30 minutes, and either Stat3 (79D7) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP® Human c-Fos Promoter Primers #4663, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2523   Acetyl-Stat3 (Lys685) Antibody
34911   Phospho-Stat3 (Ser727) (D4X3C) Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack
8768   Stat3α (D1A5) XP® Rabbit mAb
9131   Phospho-Stat3 (Tyr705) Antibody
9133   Stat1/2/3/5 Control Cell Extracts
9134   Phospho-Stat3 (Ser727) Antibody
9136   Phospho-Stat3 (Ser727) (6E4) Mouse mAb
9138   Phospho-Stat3 (Tyr705) (3E2) Mouse mAb
9139   Stat3 (124H6) Mouse mAb
9145   Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb
94994   Phospho-Stat3 (Ser727) (D8C2Z) Rabbit mAb
98543   Phospho-Stat3 (Ser754) Antibody

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.


Stat3 (79D7) Rabbit mAb

Immune Cell Signaling Pathways