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#4547 Phospho-PDGF Receptor α (Tyr1018) Antibody

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年8月23日15時25分 現在
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#4547S100 μL66,000
#4547T20 μL39,000
Custom Antibody Sampler Kitの構成品を選択できます。

PDGFRA 製品一覧 | #4547 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
内在性190 Rabbit-20℃
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス -
4547 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)


内在性レベルのTyr1018 がリン酸化されたPDGFR αタンパク質を検出します。活性型のPDGFR βタンパク質とは交差しませんが、EGFR などのチロシンがリン酸化された他のタンパク質チロシンキナーゼと交差反応する可能性があります。
ヒトのPDGFR αタンパク質のTyr1018 周辺領域 (合成リン酸化ペプチド)

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※下記の社内データは、すべて4547 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of cell extracts from NIH/3T3 cells, untreated or treated with PDGF-AA, using Phospho-PDGF Receptor α (Tyr1018) Antibody (upper) or PDGF Receptor α Antibody #3164 (lower).


Platelet derived growth factor (PDGF) family proteins exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ). PDGFRα and PDGFRβ share 75% to 85% sequence homology between their two intracellular kinase domains, while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGFRα homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRβ homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor α/β binds PDGF B, C, and D homodimers, as well as the PDGF AB heterodimer (2). PDGFRα and PDGFRβ can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLCγ, and NCK. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation (5). Tyr751 in the kinase-insert region of PDGFRβ is the docking site for PI3 kinase (6). Phosphorylated pentapeptides derived from Tyr751 of PDGFRβ (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRβ (7). Tyr740 is also required for PDGFRβ-mediated PI3 kinase activation (8).

Phosphorylation of PDGFRα on Tyr1018 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery. For more information visit www.phosphosite.org, CST's expert curated modfication site knowledge base.

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2227   Phospho-PDGF Receptor β (Tyr1021) (6F10) Rabbit mAb
2992   Phospho-PDGF Receptor α (Tyr754) (23B2) Rabbit mAb
3124   Phospho-PDGF Receptor β (Tyr1009) (42F9) Rabbit mAb
3161   Phospho-PDGF Receptor β (Tyr751) Antibody
3162   PDGF Receptor β Antibody
3164   PDGF Receptor α Antibody
3166   Phospho-PDGF Receptor β (Tyr751) (88H8) Mouse mAb
3168   Phospho-PDGF Receptor β (Tyr740) (32A9) Rabbit mAb
3169   PDGF Receptor β (28E1) Rabbit mAb
3170   Phospho-PDGF Receptor α (Tyr849)/PDGF Receptor β (Tyr857) (C43E9) Rabbit mAb
3173   Phospho-PDGF Receptor β (Tyr771) (76D6) Rabbit mAb
3174   PDGF Receptor α (D1E1E) XP® Rabbit mAb
3175   PDGF Receptor β (2B3) Mouse mAb
4549   Phospho-PDGF Receptor β (Tyr751) (C63G6) Rabbit mAb
4564   PDGF Receptor β (C82A3) Rabbit mAb
5241   PDGF Receptor α (D13C6) XP® Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack

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Phospho-PDGF Receptor α (Tyr1018) Antibody

Immune Cell Signaling Pathways