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#4499 Histone H3 (D1H2) XP® Rabbit mAb

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年11月13日15時20分 現在
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#4499S100 μL61,000
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#4499L300 μL146,000
#4499T20 μL39,000
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HISTONE H3 製品一覧 | #4499 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
内在性17Rabbit IgG-20℃
種交差性 (社内試験済)
交差する可能性がある種 i

社内試験はしていませんが、配列が100%相同であるため反応すると推定される種

ヒト、マウス、ラット、サル ハムスター、ニワトリ、キイロショウジョウバエ、アフリカツメガエル、ゼブラフィッシュ、ウシ
4499 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。
推奨プロトコールはCST社内試験の徹底的なバリデーションに基づいて作成されておりますので、正確かつ再現性の高い結果が得られます。

注:各製品に最適化されたプロトコールをリンクしています。

 

ウェスタンブロッティング (1:2000)免疫組織染色 (パラフィン) (1:400)免疫蛍光細胞染色 (IF-IC) (1:400)フローサイトメトリー (1:50)

下記ステップについては、データシートの右側もあわせてご参照ください。

IHC-P: 抗体希釈液 / 抗原賦活化

CST推奨プロトコールに欠かせない関連製品

特異性・感度
内在性レベルのHistone H3.1、H3.2、H3.3 アイソフォームを含むHistone H3 タンパク質を検出します。また、Histone H3 variant CENP-A タンパク質も検出します。他のCore Histone タンパク質とは交差しません。
使用抗原
ヒトのHistone H3 タンパク質のC末端領域 (合成ペプチド)

ホモロジー (相同性) 検索をご希望の場合 >>>

ホモロジー検索をご要望の際は、ご希望のサンプル種のアミノ酸配列とともにお問合せください。

社内データ

※下記の社内データは、すべて4499 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Histone H3 (D1H2) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Histone H3 (D1H2) XP® Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Histone H3 (D1H2) XP® Rabbit mAb (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red).


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of human peripheral blood lymphocytes using Histone H3 (D1H2) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.

バックグラウンド

Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

使用例
 
関連製品
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12757   SignalFire™ Elite ECL Reagent
13079   Immunohistochemistry Application Solutions Kit (Rabbit)
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13939   Symmetric Di-Methyl Histone H3 (Arg8) (E1W5H) Rabbit mAb
13969   Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb
13998   Acetyl-Histone H3 (Lys18) (D8Z5H) Rabbit mAb
14111   Mono-Methyl-Histone H3 (Lys36) (D9J1D) Rabbit mAb
14186   Mono-Methyl-Histone H3 (Lys9) (D1P5R) Rabbit mAb
14932   Acetyl-Histone H3 (Lys23) (D6Y7M) Rabbit mAb
2186   CENP-A Antibody
2578   Histone H2A Antibody II
27683   Acetyl-Histone H3 (Lys36) (D9T5Q) Rabbit mAb
2901   Di-Methyl-Histone H3 (Lys36) (C75H12) Rabbit mAb
2934   Histone H2B (53H3) Mouse mAb
2935   Histone H4 (L64C1) Mouse mAb
3377   Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb
3636   Histone H2A (L88A6) Mouse mAb
4243   Acetyl-Histone H3 (Lys56) Antibody
4260   Tri-Methyl-Histone H3 (Lys79) Antibody
4353   Acetyl-Histone H3 (Lys27) Antibody
4473   Pan-Methyl-Histone H3 (Lys9) (D54) XP® Rabbit mAb
4658   Di-Methyl-Histone H3 (Lys9) (D85B4) XP® Rabbit mAb
4909   Tri-Methyl-Histone H3 (Lys36) (D5A7) XP® Rabbit mAb
5326   Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb
53348   Phospho-Histone H3 (Ser10) (D7N8E) XP® Rabbit mAb
5427   Di-Methyl-Histone H3 (Lys79) (D15E8) XP® Rabbit mAb
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
74073   Tri-Methyl-Histone H3 (Lys79) (E8B3M) Rabbit mAb
7627   Acetyl-Histone H3 (Lys14) (D4B9) Rabbit mAb
7727   Biotinylated Protein Ladder Detection Pack
8059   SignalStain® DAB Substrate Kit
8112   SignalStain® Antibody Diluent
8114   SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
8173   Acetyl-Histone H3 (Lys27) (D5E4) XP® Rabbit mAb
84932   Mono-Methyl-Histone H3 (Lys27) (D3R8N) Rabbit mAb
9398   Mono-Methyl-Histone H3 (Lys79) Antibody
95191   Mono/Di-Methyl Histone H3 (Lys56) (D4L7L) Rabbit mAb
9649   Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb
9675   Acetyl-Histone H3 (Lys18) Antibody
9677   Acetyl-Histone H3 (Lys9/Lys14) Antibody
9701   Phospho-Histone H3 (Ser10) Antibody
9707   Methyl-Histone H3 (Arg2) Antibody
9711   Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody
9713   Phospho-Histone H3 (Ser28) Antibody
9714   Phospho-Histone H3 (Thr3) Antibody
9723   Mono-Methyl-Histone H3 (Lys4) Antibody
9725   Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb
9727   Tri-Methyl-Histone H3 (Lys4) Antibody
9728   Di-Methyl-Histone H3 (Lys27) (D18C8) XP® Rabbit mAb
9733   Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb
9751   Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb
9753   Di-Methyl-Histone H3 (Lys9) Antibody
9763   Tri-Methyl-Histone H3 (Lys36) Antibody
9764   Phospho-Histone H3 (Thr11) Antibody
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XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

本製品は試験研究用です。

Histone H3 (D1H2) XP® Rabbit mAb

Immune Cell Signaling Pathways

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