CST logo

#4408 Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)

 
  • Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) Data Sheet PDF
CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年12月6日15時40分 現在
ご登録代理店情報 i
カスタマー情報にご登録いただいた代理店を表示しています。
ご登録代理店の変更はこちら
#4408S250 μL25,000
ログインすると国内在庫状況がご確認いただけます。

IGG 製品一覧

由来
ヤギ
用途 (希釈倍率)
最適な希釈率はアプリケーションごとに決定する必要があります。免疫蛍光染色とフローサイトメトリーでは1:250 - 1:1000 で良好な結果が得られます。
情報
Anti-Mouse IgG (H+L), F(ab')2 Fragment を最適条件下でAlexa Fluor® 488 標識し、濃度を2 mg/mL に調整しました。ヒトのIgG と血清にて吸収してあります。
貯法
4℃
社内データ

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb #2966 detected with Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) (green) compared to a nonspecific negative control antibody (red).

High Content Screening

High Content Screening

High content analysis of A439 cells exposed to varying concentrations of caffeine for 30 min prior to and 1.5 hr following a 100 mJ UV-treatment. With increasing concentrations of caffeine, a significant decrease (~2.5 fold) in phospho-p53 signal as compared to the UV-treated control was observed. When using phospho-p53 as a measurement, the IC50 of this compound was 2.95 mM. Data was generated on the Acumen® HCS platform using Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate).

IF-IC

IF-IC

Confocal immunofluorescent analysis of mouse embryonic stem cells growing on mouse embryonic fibroblast (MEF) feeder cells using SSEA1 (MC480) Mouse mAb #4744 detected with anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


バックグラウンド

This product has been optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

使用例
 
関連製品
4084   DRAQ5®
4409   Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)
4410   Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)
4412   Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)
4413   Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)
4414   Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)
4416   Anti-rat IgG (H+L), (Alexa Fluor® 488 Conjugate)
4417   Anti-rat IgG (H+L), (Alexa Fluor® 555 Conjugate)
4418   Anti-rat IgG (H+L), (Alexa Fluor® 647 Conjugate)
4753   Cellular Localization IF Antibody Sampler Kit
8889   Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate)
8890   Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate)

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation, for research use only for immunocytometry, immunohistochemistry, high content screening (HCS) analysis, or flow cytometry applications.
Acumen is a registered trademarks of TTP Labtech.
DRAQ5 is a registered trademark of Biostatus Limited.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for Immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

本製品は試験研究用です。

Anti-Mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)

Metabolic Reprogramming in Disease

お薦めリンク

テクニカルサポート

製品特集

Applications