#4351 CHD1 (D8C2) Rabbit mAb
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PhosphoSitePlus®：
| CSTコード | 包装 | 希望納入価格 (円) |
国内在庫  2019年12月13日15時35分 現在 |
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| #4351S | 100 μL | 56,000 | ログインすると国内在庫状況がご確認いただけます。
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| 感度 | 分子量 (kDa) | 抗体の由来 | 貯法 | |
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| 内在性 | 220 | Rabbit IgG | -20℃ |
| 種交差性 (社内試験済) |
交差する可能性がある種
社内試験はしていませんが、配列が100%相同であるため反応すると推定される種 |
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| ヒト、マウス、ラット、サル | ウシ、イヌ、ブタ |
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4351 の推奨プロトコール 最適な結果を得るために：Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。 注：各製品に最適化されたプロトコールをリンクしています。 |
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ウェスタンブロッティング (1:1000)、免疫沈降 (1:50)、クロマチン免疫沈降 (1:100)
| 特異性・感度 | |
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| 内在性レベルのCHD1 タンパク質を検出します。 |
| 使用抗原 | |
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| ヒトのCHD1 タンパク質のC末端近傍領域 (合成ペプチド) |
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| 社内データ |
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※下記の社内データは、すべて4351 の推奨プロトコールで実験した結果です。
Western Blotting
Western blot analysis of extracts from various cell lines using CHD1 (D8C2) Rabbit mAb.
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from mES cells and either CHD1 (D8C2) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse Oct-4 Promoter Primers #4653, SimpleChIP® Mouse RPL30 Intron 2 Primers #7015, and SimpleChIP® Mouse MYT-1 Promoter Primers #8985. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
| バックグラウンド |
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Chromodomain-helicase-DNA-binding domain (CHD) proteins have been identified in a variety of organisms (1,2). This family of proteins, which consists of nine members, has been divided into three separate subfamilies: subfamily I (CHD1 and CHD2), subfamily II (CHD3 and CHD4), and subfamily III (CHD5, CHD6, CHD7, CHD8, and CHD9). All of the CHD proteins contain two tandem N-terminal chromodomains, a SWI/SNF-related ATPase domain, and a C-terminal DNA binding domain (1,2). The chromodomains facilitate binding to methylated lysine residues of histone proteins and confer interactions with specific regions of chromatin. The SWI/SNF-related ATPase domain utilizes the energy from ATP hydrolysis to modify chromatin structure. CHD1 is a euchromatic protein that associates with the promoters of active genes, and is required for the maintenance of open chromatin and pluripotency in embryonic stem cells (3-6). The two chromodomains of CHD1 facilitate its recruitment to active genes by binding to methyl-lysine 4 of histone H3, a mark associated with transcriptional activation (4-6). Yeast CHD1 is a component of the SAGA and SLIK histone acetyltransferase complexes, and is believed to link histone methylation with histone acetylation during transcriptional activation (6). The CHD2 protein is not well characterized; however, mouse knockout studies suggest important functions in development and tumor suppression. Homozygous CHD2 knockout mice exhibit delayed growth and perinatal lethality (7). Heterozygous knockout mice show increased mortality and gross organ abnormalities, in addition to increased extramedullary hematopoiesis and susceptibility to lymphomas (7,8). CHD2 mutant cells are defective in hematopoietic stem cell differentiation and exhibit aberrant DNA damage responses (8).
- Hall, J.A. and Georgel, P.T. (2007) Biochem Cell Biol 85, 463-76.
- Marfella, C.G. and Imbalzano, A.N. (2007) Mutat Res 618, 30-40.
- Gaspar-Maia, A. et al. (2009) Nature 460, 863-8.
- Sims, R.J. et al. (2005) J Biol Chem 280, 41789-92.
- Flanagan, J.F. et al. (2005) Nature 438, 1181-5.
- Pray-Grant, M.G. et al. (2005) Nature 433, 434-8.
- Marfella, C.G. et al. (2006) J Cell Physiol 209, 162-71.
- Nagarajan, P. et al. (2009) Oncogene 28, 1053-62.
| 使用例 |
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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
本製品は試験研究用です。
