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#4243 Acetyl-Histone H3 (Lys56) Antibody

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2020年2月28日15時15分 現在
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#4243S100 μL67,000
#4243T20 μL39,000
Custom Antibody Sampler Kitの構成品を選択できます。

HISTONE H3 製品一覧 | #4243 が入っているAntibody Sampler キット一覧

感度分子量 (kDa)抗体の由来貯法
種交差性 (社内試験済)
交差する可能性がある種 i


ヒト、マウス、ラット、サル -
4243 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)


内在性レベルのLys56 がアセチル化されたHistone H3 タンパク質を検出します。Lys9、14、18、27 がアセチル化されたHistone H3 タンパク質とは交差しません。
Lys56 がアセチル化されたHistone H3 タンパク質のN末端周辺領域 (合成ペプチド)

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※下記の社内データは、すべて4243 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, C6 and COS cells, untreated or treated with Trichostatin A (TSA) #9950 (400 nM for 18 h), using Acetyl-Histone H3 (Lys56) Antibody (upper) and Histone H3 Antibody #9715 (lower).



Acetyl-Histone H3 (Lys56) Antibody specificity was determined by peptide ELISA. The graph depicts the binding of the antibody to pre-coated acetyl-histone H3 (Lys56) peptide in the presence of increasing concentrations of various competitor peptides. As shown, only the acetyl-histone H3 (Lys56) peptide competed away binding of the antibody.


Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

Acetylation of histone H3 on Lys56 is critical for proper packaging of DNA into chromatin during DNA replication and DNA damage repair (12-14). Histone H3 is acetylated on Lys56 by CBP and p300 in response to DNA damage induced by treatment of cells with γ radiation, ultraviolet light, MMS, or hydroxyurea (14). Following DNA damage, chromatin assembly factor 1 protein (CAF-1) incorporates acetylated histones into chromatin at sites of DNA repair (14). The class III histone deacetylases (HDACs) SirT1, SirT2 and SirT6 have been shown to deacetylate histone H3 at Lys56 (14,15); however, treatment of cells with sodium butyrate or trichostatin A also leads to increased acetylation, implicating a class I or class II HDAC as an additional histone H3 Lys56 deacetylase (14). Histone H3 Lys56 acetylation is high in multiple types of cancer, and acetylation levels directly correlate with cellular dedifferentiation and tumorigenicity (14).

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
2028   SirT1 Antibody (Mouse Specific)
2493   SirT1 (D739) Antibody
2590   SirT6 Antibody
2595   Histone H2A.X Antibody
2650   Histone H3 Antibody (ChIP Formulated)
2902   ASF1B (C70E2) Rabbit mAb
2990   ASF1A (C6E10) Rabbit mAb
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4620   Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated)
6883   SignalFire™ ECL Reagent
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9715   Histone H3 Antibody
9717   Histone H3 (3H1) Rabbit mAb
9718   Phospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb

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Acetyl-Histone H3 (Lys56) Antibody

Immune Cell Signaling Pathways