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#4206 Phospho-NMDA Receptor 2A (GluN2A) (Tyr1246) Antibody

CSTコード 包装
希望納入価格 (円)
国内在庫 i
2019年12月11日15時25分 現在
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#4206S100 μL66,000

NMDAR2A 製品一覧

感度分子量 (kDa)抗体の由来貯法
種交差性 (社内試験済)
交差する可能性がある種 i


ラット ヒト、マウス
4206 の推奨プロトコール i

最適な結果を得るために:Cell Signaling Technology (CST) 社は、各製品の推奨プロトコールを使用することを強くお薦めいたします。



ウェスタンブロッティング (1:1000)免疫沈降 (1:50)


内在性レベルのTyr1246 がリン酸化されたNMDA Receptor 2A (GluN2A) タンパク質を検出します。Tyr1252 がリン酸化されたNMDA Receptor 2B (GluN2B) タンパク質とも交差する可能性があります。
ヒトのNMDA Receptor 2A (GluN2A) のTyr1246 周辺領域 (合成リン酸化ペプチド)

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※下記の社内データは、すべて4206 の推奨プロトコールで実験した結果です。

Western Blotting

Western Blotting

Western blot analysis of extracts from rat brain, either shamoperated or 15 min ischemia, using Phospho-NMDA Receptor 2A (GluN2A) (Tyr1246) Antibody (upper) or NMDA Receptor 2A (GluN2A) Antibody (lower)


N-methyl-D-aspartate receptor (NMDAR) forms a heterodimer of at least one NR1 and one NR2A-D subunit. Multiple receptor isoforms with distinct brain distributions and functional properties arise by selective splicing of the NR1 transcripts and differential expression of the NR2 subunits. NR1 subunits bind the co-agonist glycine and NR2 subunits bind the neurotransmitter glutamate. Activation of the NMDA receptor or opening of the ion channel allows flow of Na+ and Ca2+ ions into the cell, and K+ out of the cell (1). Each subunit has a cytoplasmic domain that can be directly modified by the protein kinase/phosphatase (2). PKC can phosphorylate the NR1 subunit (NMDAR1) of the receptor at Ser890/Ser896, and PKA can phosphorylate NR1 at Ser897 (3). The phosphorylation of NR1 by PKC decreases its affinity for calmodulin, thus preventing the inhibitory effect of calmodulin on NMDAR (4). The phosphorylation of NR1 by PKA probably counteracts the inhibitory effect of calcineurin on the receptor (5). NMDAR mediates long-term potentiation and slow postsynaptic excitation, which play central roles in learning, neurodevelopment, and neuroplasticity (6).

EphrinB2 binding to the receptor EphB leads to the activation of Src family tyrosine kinases, which phosphorylate NMDAR2B at Tyr1252, Tyr1336 and Tyr1472. In turn, phosphorylated NMDAR2B enhances the ability of the functional NMDA receptor to regulate Ca2+ influx in response to glutamate (7). The phosphorylation site of NMDAR2A at Tyr1246 is the conserved site of NMDAR2B at Tyr1252 and was independently identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of NMDAR2A at Tyr1246 was observed in extracts isolated from ischemic rat brain. For additional information please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org.

12630   SignalFire™ Plus ECL Reagent
12757   SignalFire™ Elite ECL Reagent
3356   Phospho-CaMKII (Tyr231) Antibody
3381   Phospho-NMDA Receptor 1 (GluN1) (Ser890) Antibody
4205   NMDA Receptor 2A (GluN2A) Antibody
4781   Phospho-PKA C (Thr197) Antibody
4782   PKA C-α Antibody
6883   SignalFire™ ECL Reagent
7074   Anti-rabbit IgG, HRP-linked Antibody
7727   Biotinylated Protein Ladder Detection Pack

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Phospho-NMDA Receptor 2A (GluN2A) (Tyr1246) Antibody

Metabolic Reprogramming in Disease